Effect of curcumin in the prevention of experimentally induced nephrolithiasis in rats by ethylene glycol and Vitamin D3

다국어 초록 (Multilingual Abstract)

Curcumin (CMN) is known to have beneficial role in anorexia, coryza, cough, diabetic wounds,
and hepatic disorders apart from its inherent antioxidant effects. Therefore, the present study was
aimed to evaluate antioxidant effect of CMN in prevention of nephrolithiasis in rats-induced by
ethylene glycol (EG) and Vitamin D3 (Vit. D3). Male Wistar rats (175 - 200 g) were randomized in
groups like control, EG + Vit. D3 induced nephrolithiatiatic rats, CMN treated rats, CMN + EG +
Vit. D3 treated rats, Vit. E + EG + Vit. D3 treated rats. Urine was collected weekly throughout the
experimental protocol and estimated for calcium oxalate (CaO) count. After completion of
experimental protocol serum was estimated for blood urea nitrogen and creatinine. Both the
kidneys were excised and used to evaluate levels of biomarkers of oxidative stress and calcium
oxalate crystal deposition by histopathological studies. Administration of EG and Vit. D3 to rats
resulted in increased oxidative stress, hyperoxaluria and renal deposition of CaO crystals.
Supplementation with CMN improves kidney function, reduces elevated oxidative stress, urinary
oxalate level and renal deposition of CaO which shows its protective action in nephrolithiasis.
The increased deposition of stone in the kidney and stone forming constituents of nephrolithiatic
rats were effectively lowered by treatment of CMN.

다국어 초록 (Multilingual Abstract)

Curcumin (CMN) is known to have beneficial role in anorexia, coryza, cough, diabetic wounds,
and hepatic disorders apart from its inherent antioxidant effects. Therefore, the present study was
aimed to evaluate antioxidant effect of CMN in prevention of nephrolithiasis in rats-induced by
ethylene glycol (EG) and Vitamin D3 (Vit. D3). Male Wistar rats (175 - 200 g) were randomized in
groups like control, EG + Vit. D3 induced nephrolithiatiatic rats, CMN treated rats, CMN + EG +
Vit. D3 treated rats, Vit. E + EG + Vit. D3 treated rats. Urine was collected weekly throughout the
experimental protocol and estimated for calcium oxalate (CaO) count. After completion of
experimental protocol serum was estimated for blood urea nitrogen and creatinine. Both the
kidneys were excised and used to evaluate levels of biomarkers of oxidative stress and calcium
oxalate crystal deposition by histopathological studies. Administration of EG and Vit. D3 to rats
resulted in increased oxidative stress, hyperoxaluria and renal deposition of CaO crystals.
Supplementation with CMN improves kidney function, reduces elevated oxidative stress, urinary
oxalate level and renal deposition of CaO which shows its protective action in nephrolithiasis.
The increased deposition of stone in the kidney and stone forming constituents of nephrolithiatic
rats were effectively lowered by treatment of CMN.

참고문헌 (Reference)

1 Giuseppe M, "The relationship of 30 vitamin D receptor haplotypes to urinary supersaturation of calcium oxalate salts and to age at onset and familial prevalence of nephrolithiasis" 19 : 2259-2265, 2004

2 Hayrettin O, "The effect of L-arginine methyl ester on indices of free radical involvement in a rat model of experimental nephrocalcinosis" 34 : 305-314, 2006

3 Yamaguchi S, "Study of a rat model for calcium oxalate crystal formation without severe renal damage in selected conditions" 12 : 290-298, 2005

4 Veena, SP, Preetha, "Renal peroxidative changes mediated by oxalate: The protective role of fucoidan" 7 : 1789-1795, 2006

5 Itoh Y, "Preventive effects of green tea on renal stone formation and the role of oxidative stress in nephrolithiasis" 173 : 271-275, 2005

6 Jonassen JA, "Oxalate-induced changes in the viability and growth of human renal epithelial cells" 10 : 446-451, 1999

7 John AS, "Nephrocalcinosis: molecular insights into calcium precipitation within the kidney" 106 : 549-561, 2004

8 Chintan G, "Nebivolol Reduces Experimentally Induced Warm Renal Ischemia Reperfusion Injury in Rats" 30 : 921-930, 2008

9 Khand FD, "Mitochondrial superoxide production during oxalatemediated oxidative stress in renal epithelial cells" 32 : 1339-1350, 2002

10 Hoppe B, "Metabolic disorders and molecular background of urolithiasis in childhood" 13 : 267-280, 1999

11 Palaninathan V, "Low molecular weight heparin protection against oxalate-induced oxidative renal insult" 370 : 108-114, 2006

12 Thamilselvan S, "Lipid peroxidation in ethylene glycol induced hyperoxaluria and calcium oxalate nephrolithiasis" 157 : 1059-1063, 1997

13 Fredric LC, "Kidney stone disease" 115 : 2598-2608, 2005

14 Xiao QL, "Increased Intestinal Vitamin D Receptor in Genetic Hypercalciuric Rats A Cause of Intestinal Calcium Hyperabsorption" 91 : 661-667, 1993

15 Jie F, "Impact of ammonium chloride administration on a rat ethylene glycol urolithiasis model" 13 : 299-306, 1999

16 Sivagnanam T, "Free radical scavengers, catalase and superoxide dismutase provide protection from oxalate-associated injury to llc-pk1 and mdck cells" 164 : 224-229, 2000

17 Shahid RK, "Experimental calcium oxalate nephrolithiasis in the rat. Role of the renal papilla" 107 : 59-69, 1982

18 de Bruijn WC, "Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats" 8 : 541-549, 1994

19 Harry H, Abraham, "Estimation of Creatinine by the Jaffe Reaction A Comparison of Three Methods" 14 : 222-238, 1968

20 Halabea A, "Effect of vitamin D3 on the conversion of ethylene glycol to glycolate and oxalate in ethylene glycol-fed rats" 330 : 135-139, 2003

21 Balasubramanyam M, "Curcumininduced inhibition of cellular reactive oxygen species generation: Novel therapeutic implications" 28 : 715-721, 2003

22 Naveen T, "Curcumin, a diferuloylmethane, attenuates cyclosporineinduced renal dysfunction and oxidative stress in rat kidneys" 5 : 1-10, 2005

23 Tawashi R, "Crystallisation of calcium oxalate dihydrate in normal urine in presence of sodium copper chlorophyllin" 10 : 173-176, 1982

24 Shukkur MF, "Credentials of Spirulina diet on stability and flux related properties on the biomineralization process during oxalate mediated renal calcification in rats" 24 : 932-942, 2005

25 Ho-shiang H, "Changes in the oxidantantioxidant balance in the kidney of rats with nephrolithiasis induced by ethylene glycol" 167 : 2584-2593, 2002

26 Huang HS, "Changes in the Oxidant-Antioxidant Balance in the Kidney of Rats With Nephrolithiasis Induced by Ethylene Glycol" 167 : 2584-2593, 2002

27 de Water R, "Calcium oxalate nephrolithiasis: effect of renal crystal deposition on the cellular composition of the renal interstitium" 4 : 761-771, 1999

28 Coothan K, "Beneficial role of sulfated polysaccharides from edible seaweed Fucus vesiculosus in experimental hyperoxaluria" 100 : 1552-1559, 2007

29 Ayantika G, "Anti-oxidative Effect of a Protein from Cajanus indicus L against Acetaminophen-induced Hepato-nephro Toxicity" 40 : 1039-1049, 2007