독활지황탕(獨活地黃湯)이 βA로 유도된 Alzheimer`s Disease 병태 모델에 미치는 영향 = The Effects of Dokhwaljihwang-tang(Duhuodihuangtang) on the Alzheimer`s Disease Model Induced by βA

다국어 초록 (Multilingual Abstract)

Objectives: This research investigates the effect of the DHJHT extract on Alzheimer`s disease. Specifically, the effects of the DHJHT extract on (1) the behavior (2) the infarction area of the hippocampus, and brain tissue injury in AD mice induced with βA were investigated. Methods: The effects of the DHJHT extract on the proinflammation cytokines mRNA expression and production of BACE, APP and βA in in BV2 microglial cell line treated by lipopolysacchaide(LPS) plus βA were investigated. The effects of the DHJHT extract on the behavior of the memory deficit mice induced by scopolamine were investigated. Results: 1. The DHJHT extract suppressed the expression of IL-1β, IL-6, TNF-α, COX-2, and NOS-II, BACE and APP mRNA in BV2 microglial cell line treated by LPS plus βA. 2. The DHJHT extract suppressed the expression of βA production in BV2 microglial cell line treated with LPS plus βA. 3. The DHJHT extract showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. 4. The DHJHT group suppressed the expression of IL-1β, TNF-α, MDA, and CD68+/CD11b+ in the brain tissue of the mice with AD induced by βA. 5. The DHJHT group reduced the infarction area of hippocampus, and controlled the injury of the brain tissue in the mice with AD induced by βA. 6. The DHJHT group reduced tau protein, and GFAP in the brain tissue of the mice with AD induced by βA. Conclusions: These results suggest that the DHJHT group may be effective for the treatment of AD. Thus, DHJHT could be considered among the future therapeutic drugs indicated for the treatment of AD.

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