Genetic Variability of Sorghum Charcoal Rot Pathogen (Macrophomina phaseolina) Assessed by Random DNA Markers

다국어 초록 (Multilingual Abstract)

Genetic diversity among selected isolates of Macrophomina
phaseolina, a causal agent of charcoal rot
(stalk rot) disease in sorghum was studied using PCRRAPD
markers. A set of ten isolates, from ten different
rabi sorghum genotypes representing two traditional
sorghum growing situations viz., Dharwad- a transitional
high rainfall region and Bijapur- a semi-arid low
rainfall region in South India. From a set of 40 random
primers tested, amplicon profiles of 15 were reproducible.
A total of 149 amplicon levels, with an average of
9.9 bands per primer, were available for analysis, of
which 148 were polymorphic (99.3%). It was possible to
discriminate all the isolates with any of the 15 primers
employed. UPGMA clustering of data indicated that the
isolates shared varied levels of genetic similarity within
a range of 0.14 to 0.72 similarity coefficient index and it
was suggestive that grouping of isolates was not related
to sampling location in anyway. A high level of genetic
heterogeneity of 0.28 was recorded among the isolates.

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