한천분해세균 Agarivorans sp. JS-1의 분리 및 β-아가라제의 특성 규명 = Isolation of Agarivorans sp. JS-1 and Characterization of Its β-Agarase

다국어 초록 (Multilingual Abstract)

This report looks at an agar-degrading marine bacterium and characterization of its agarase. Agar-degrading marine bacterium JS-1 was isolated with Marine agar 2216 media from seawater from the seashore of Sojuk-do, Changwon in Gyeongnam Province, Korea. The agar-degrading bacterium was named as Agarivorans sp. JS-1 by phylogenetic analysis based on 16S rRNA gene sequencing. The extracellular agarase was prepared from the culture media of Agarivorans sp. JS-1 and used for characterization. Relative activities at 20°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, and 60°C were 70%, 74%, 78%, 83%, 87%, 100%, 74%, and 66%, respectively. Relative activities at pH 5, 6, 7, and 8 were 91%, 100%, 90%, and 89%, respectively. Its extracellular agarase showed maximum activity (207 units/l) at pH 6.0 and 50°C in 20 mM Tris-HCl buffer. The residual activity after heat treatment at 20°C, 30°C, and 50°C for 30 minutes was 90%, 70%, and 50% or more, respectively. After a 2-hour heat treatment at 20°C, 30°C, 35°C, 40°C, and 45°C, the residual activity was 80%, 68%, 65%, 63%, and 57%, respectively. At 50°C and above, after heat treatment for 30 minutes, the residual activity was below 60%. Thin layer chromatography analysis suggested that Agarivorans sp. JS-1 produces extracellular β-agarases as they hydrolyze agarose to produce neoagarooligosaccharides such as neoagarohexaose (20.6%), neoagarotetraose (58.5%), and neoagarobiose (20.9%). Agarivorans sp. JS-1 and its thermotolerant β-agarase would be useful in the production of neoagarooligosaccharides, showing functional activity such as inhibition of bacterial growth and delay of starch degradation.

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