배반엽성세포를 이용한 카이메라 닭 ( 화이트레그혼 ↔ 오골계 ) 생산 = Production of Chimeric Chickens ( White Leghorn ↔ Korean Native Ogol ) by Blastodermal Cell Transfer

다국어 초록 (Multilingual Abstract)

The chicken embryo contains 30,000 to 60,000 cells at development stage X, the blastodermal cells at this stage are believed to he pluripotent and subsequently contribute to melanocytes derived from the neural crest, to erythrocytes and to germ cells. In this study, blastodermal cells were isolated from stage X embryos of Korean Native Ogol Chicken (KNOC). The isolated blastodemial cells were transfected with liposome and dispersed by pipetting. A 2㎕ volume of cell suspension was microinjected into the subgerminal cavity of recipient embryos at an equivalent stage of development of the donor and recipient. Of the 332 White Leghorn embryos that were injected with KNOC blastodermal cells, three unhitched embryos were phenotypically chimeric with respect to feather color and one embryo hatched. This technique may be useful in development of a transgenic chicken production system.