갈겨니 ( Zacco temmincki ) 의 진화에 관한 연구 Ⅷ. 피라미아과 ( subamily Danioninae ) 어류의 mitochondrial DAN 비교 = Evolutionary Study on the Dark Chub ( Zacco Temmincki ) Ⅷ. Mitochondrial DNA Analysis of the Subfamily Danioninae ( Pisces , Cyprinidae )

다국어 초록 (Multilingual Abstract)

Mitochondrial DNAs(mtDNAs) from four species (Zacco temmincki(MM and MS allelotypes), Z. platypus, Opsariichthys bidens, and Aphyocypris chinensis) of subfamily Danioninae inhabiting in Korean fresh water were digested with each of 9 restriction endonucleases. No differences among four species were detected in total genome size (about 16.7±0.5Kb) of the mtDNA. The typical patterns of mtDNA fragments of each species were observed in digestion profiles produced by Bgl I, Bst EII and Xba I enzymes and these ones were thought to e as a genetic marker in mtDNA. Most of the restriction fragments were homogeneous in the intraspecies, but restriction fragment length polymorphism were discovered by Bcl I and Xba I in Z. temmincki (MS type). These polymorphisms may be generated by loss or gain of a restriction enzyme recognition site in point mutations. The comparison of restriction fragment patterns permitted the estimation of fragment homology (F) and nucleotide sequence divergence (p). The sequence divergence (p) between two types of Z. temmincki was 0.042, and 0.107 (range 0.082-0.133) between the congeneric species. The mean p value between genera Zacco except Z. platypus and Opsariichthys bidens where they are sympatric was less than within the genera (p=0.070). The recognition site of Pst I by double digestion was nearly identical in Z. platypus and O. bidens. The nucleotide sequence divergence by restriction enzyme analysis clearly distinguishes not only intra and interspecific variations but intergeners.