The Stability of a complex between Poly G and Poly C is determined by measurement of the thermal stability of the complex, e.g. the transition temperature(Tc).
The Values for Tc are varied with ionic strength and ionic environment. the stability of t...
The Stability of a complex between Poly G and Poly C is determined by measurement of the thermal stability of the complex, e.g. the transition temperature(Tc).
The Values for Tc are varied with ionic strength and ionic environment. the stability of the complex also decreases on acylation. Acetylation of 2-amino group of guanine sharply decreases Tc in the thermal hyper-chromicity transition of the Watson & Crick guanine-cytosine hydrogen bond and stacking interaction
The Stability of Poly G-Oligo C complex is estimated using Ising Model, by the measurement of Tc Vs. Concentration, Tc Vs. degree of polymerization of Oligomer. Relative stability of hydrogen bond and stacking interaction proves to be guanine - cytocine > adenine - uracil in polymer-oligomer complex.