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      KCI등재 SCIE SCOPUS

      Overexpression of PgSQS1 Increases Ginsenoside Production and Negatively Affects Ginseng Growth Rate in Panax ginseng

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      https://www.riss.kr/link?id=A82355401

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      다국어 초록 (Multilingual Abstract)

      The medicinal plant Panax ginseng (P. ginseng) contains various phytosterols and bioactive triterpene saponins (ginsenosides). Squalene synthase catalyzes the first committed step in ginsenoside biosynthesis. Transgenic plants of P. ginseng were gener...

      The medicinal plant Panax ginseng (P. ginseng) contains various phytosterols and bioactive triterpene saponins (ginsenosides). Squalene synthase catalyzes the first committed step in ginsenoside biosynthesis. Transgenic plants of P. ginseng were generated by introducing the squalene synthase gene derived from P. ginseng. Adventitious roots of the transgenic ginseng grew best in B5 medium, and 2 g of inoculum secured an optimal growth rate. Two phytohormones, indolebutyric acid and 1-naphtalene acetic acid, increased root growth and decreased ginsenoside production. Treatment with two selected elicitors, chitosan and jasmonic acid, and a precursor of the isoprenoid pathway, mevalonic acid, enhanced ginsenoside production and retarded ginseng growth rate.

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      목차 (Table of Contents)

      • INTRODUCTION
      • MATERIALS AND METHODS
      • RESULTS AND DISCUSSION
      • ACKNOWLEDGEMENTS
      • REFERENCES
      • INTRODUCTION
      • MATERIALS AND METHODS
      • RESULTS AND DISCUSSION
      • ACKNOWLEDGEMENTS
      • REFERENCES
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      참고문헌 (Reference)

      1 Ketchum RE, "The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate" 62 : 97-105, 1999

      2 Chappell J, "The Biochemistry and Molecular Biology of Isoprenoid Metabolism" 107 : 1-6, 1995

      3 Bloch K, "Sterol molecule: structure, biosynthesis, and function" 57 : 378-383, 1992

      4 Devarenne TP, "Regulation of squalene synthase, a key enzyme of sterol biosynthesis, in tobacco" 129 : 1095-1106, 2002

      5 Choi YE, "Rapid and efficient Agrobacterium-mediated transformation of Panax ginseng by plasmolyzing pre-treatment of cotyledons" 20 : 616-621, 2001

      6 Nam MH, "Proteome analysis of the responses of Panax ginseng C. A. Meyer leaves to high light: use of electrospray ionization quadrupole-time of flight mass spectrometry and expressed sequence tag data" 3 : 2351-2367, 2003

      7 Kim SI, "Proteome analysis of hairy root from Panax ginseng C.A. Meyer using peptide fingerprinting, internal sequencing and expressed sequence tag data" 3 : 2379-2392, 2003

      8 Kennedy MA, "Positive and negative regulation of squalene synthase (ERG9), an ergosterol biosynthetic gene, in Saccharomyces cerevisiae" 1517 : 177-189, 2001

      9 Doares SH, "Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway" 92 : 4095-4098, 1995

      10 Gamborg OL, "Nutrient requirements of suspension cultures of soybean root cells" 50 : 151-158, 1968

      1 Ketchum RE, "The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate" 62 : 97-105, 1999

      2 Chappell J, "The Biochemistry and Molecular Biology of Isoprenoid Metabolism" 107 : 1-6, 1995

      3 Bloch K, "Sterol molecule: structure, biosynthesis, and function" 57 : 378-383, 1992

      4 Devarenne TP, "Regulation of squalene synthase, a key enzyme of sterol biosynthesis, in tobacco" 129 : 1095-1106, 2002

      5 Choi YE, "Rapid and efficient Agrobacterium-mediated transformation of Panax ginseng by plasmolyzing pre-treatment of cotyledons" 20 : 616-621, 2001

      6 Nam MH, "Proteome analysis of the responses of Panax ginseng C. A. Meyer leaves to high light: use of electrospray ionization quadrupole-time of flight mass spectrometry and expressed sequence tag data" 3 : 2351-2367, 2003

      7 Kim SI, "Proteome analysis of hairy root from Panax ginseng C.A. Meyer using peptide fingerprinting, internal sequencing and expressed sequence tag data" 3 : 2379-2392, 2003

      8 Kennedy MA, "Positive and negative regulation of squalene synthase (ERG9), an ergosterol biosynthetic gene, in Saccharomyces cerevisiae" 1517 : 177-189, 2001

      9 Doares SH, "Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway" 92 : 4095-4098, 1995

      10 Gamborg OL, "Nutrient requirements of suspension cultures of soybean root cells" 50 : 151-158, 1968

      11 Aerts RJ, "Methyl jasmonate vapor increase the developmentally controlled synthesis of alkaloids in Cartharanthus and Cinchona seedlings" 5 : 635-643, 1994

      12 Briskin DP, "Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health" 124 : 507-514, 2000

      13 Gundlach H, "Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures" 89 : 2389-2393, 1992

      14 Yu KW, "Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer" 11 : 211-215, 2002

      15 Wentzinger LF, "Inhibition of squalene synthase and squalene epoxidase in tobacco cells triggers an up-regulation of 3-hydroxy-3-methylglutaryl coenzyme a reductase" 130 : 334-346, 2002

      16 Vogeli U, "Induction of sesquiterpene cyclase and suppression of squalene synthetase activities in plant cell cultures treated with fungal elicitor" 88 : 1291-1296, 1988

      17 Kushiro T, "In vitro conversion of 2,3-oxidosqualene into dammarenediol by Panax ginseng microsomes" 20 : 292-294, 1997

      18 Yu KW, "Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of ginseng (Panax ginseng C. A. Meyer)" 36 : 424-428, 2000

      19 Cui J, "Gynostemma pentaphyllum: identification of major sapogenins and differentiation from Panax species" 8 : 187-191, 1999

      20 Abe I, "Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes" 93 : 2189-2206, 1993

      21 Lee MH, "Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene" 45 : 976-984, 2004

      22 Jung JD, "Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags" 22 : 224-230, 2003

      23 Guan G, "Differential transcriptional regulation of the human squalene synthase gene by sterol regulatory element-binding proteins (SREBP) 1a and 2 and involvement of 5’ DNA sequence elements in the regulation" 273 : 12526-12535, 1998

      24 Farmer EE, "Diethyldithiocarbamic acid inhibits the octadecanoid signaling pathway for the wound induction of proteinase inhibitors in tomato leaves" 106 : 337-342, 1994

      25 Cheng LQ, "Conversion of major ginsenoside Rb1 to 20(S)-ginsenoside Rg3 by Microbacterium sp. GS514." 69 : 218-224, 2008

      26 Kim MK, "Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf" 25 : 599-606, 2006

      27 Lloyd G, "Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture" 30 : 421-427, 1980

      28 Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures" 15 : 473-497, 1962

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