HPLC에 의한 천연 식물성 기름 중 토코페롤 이성질체의 분리 및 정량에 관한 연구 = A Study on the Separation and Determination of Tocopherol Isomers in Some Vegetable Oils by High Performance Liquid Chromatography

다국어 초록 (Multilingual Abstract)

The application of HPLC to the separation and determination of tocopherol isomers in some vegetable oils has been studied. The optimum conditions for the analysis of tocopherol isomers were determined and then assay of tocopherol isomers in sample were performed.
The following conclusions have obtained from the results.
1. The optimum analytical conditions were that the mobile phase was n-hexane: isopropyl alcohol(99.5:0.5) at a flow rate of 2.0ml/min with fluorescence detector (Excitation 300nm, Emission 338nm)
2. Retention ratios relative to α-tocopherol were as follows: β-, 1.63:γ-, 1.78:and δ-, 2.91. The retention time for α-tocopherol was 3.29min.
3. Recoveries of individual tocopherol isomers were 100%, 97%, 93%, and 103%:and coefficients of variation were 0.71%, 3.7%, 7.6% and 3.5% for α-, β-, γ-, δ-tocopherol respectively.
4. Under the optimum condition, it was found that the content of tocopherol isomers in 100g portion were: 14.5mg for α-: 16.5mg for γ-in peanut, and 8mg for α-: 11.5mg for γ-: and 0.6mg for δ-in sesame, and 3.5mg for α-: 12.5mg for γ-in perilla, and 15.0mg for α-: 11.6mg for β-: 35.3mg for γ-: 2.0mg for δ-in rape seed.