Nitric Oxide production by Lipoarabinomannan of Mycobacterium tuberculosis in Dendritic cell = Dendritic cell에서 Mycobacterium tuberculosis의 Lipoarabinomannan에 의한 Nitric Oxide의 생성

다국어 초록 (Multilingual Abstract)

Mycobacterium tuberculosis is an important pathogene for human tuberculosis and is frequently found in cattle. Nitric xi (NO) production is an important host defense mechanism against microbial infection. It has been known that mannosylated lipoarabinomannan (ManLAM), a cell wall component of Mycobacterium tuberculosis induce production of NO in a macrophage cell line, J774. However, it has not been elucidated whether ManLAM cause NO production in Dendritic cell (DC). In this study, we evaluated NO production, inducible nitric oxide synthase (iNOS) expression by the treatment of ManLAM in DC. The treatment of ManLAM released NO in DC in a dose-dependent manner. The expression of iNOS was also increased by ManLAM as determined by reverse transcription polymerase chain reaction. These results suggest the possibility that ManLAM might affect DC function through NO production.

목차 (Table of Contents)

• 목차
• 영문초록 = 1
• 서론 = 2
• 재료 및 방법 = 5
• 세포주 배양 = 5
• NO 측정 = 5
• Western blot analysis = 6
• RT-PCR = 7
• 결과 = 9
• 고찰 = 14
• 참고문헌 = 16