토마토 유묘에 있어서 저온과 수분 스트레스에 대한 황산화효소의 활성 차이

국문 초록 (Abstract)

저온 또는 건조 처리에 따른 토마토 유묘의 생육과 부위별 항산화효소의 반응 양상을 분석한 결과, 토마토 유묘의 생체중은 처리 후 12일째에 대조구에 비해 각각 69.5%와 50.6% 감소하였다. SOD과 POD의 활성은 대조구에 비해 저온 또는 건조 처리에서 높은 활성을 보였는데, 저온 처리시에는 뿌리에서 더 높은 활성을 보였고 건조 처리에서는 잎과 줄기에서 높은 활성을 보였다. 이러한 결과는 동위효소의 발현양상에서도 일치하였다. OR의 활성은 저온 또는 건조 처리시 대조구보다 높은 활성을 보였는데, 잎과 줄기에서는 저온과 건조 처리간의 차이는 없었지만, 뿌리에서는 건조처리가 높은 경향을 보였다. OR 동위효소 발현양상은 저온과 건조처리시에는 OR-3 밴드가 잎에서는 발현되어 대조구와 차이가 있었지만, 줄기와 뿌리에서는 큰 차이가 없었다. PPO 활성은 잎에서는 모든 처리에서 차이가 없었지만, 줄기와 뿌리에서는 저온 또는 건조처리에서 대조구보다 높은 경향을 보였다. 특히 줄기의 PPO 활성은 저온 처리보다 건조처리에서 높았고 뿌리의 PPO 활성은 건조 처리보다 저온 처리에서 높았다. 동위효소의 발현양상에서도 건조처리에서는 줄기에서, 저온 처리에서는 뿌리에서 높은 밀도를 보여 불량 환경에 따른 부위별 반응 차이를 잘 반영해 주었다.

다국어 초록 (Multilingual Abstract)

Responses of antioxidant enzymes on chilling and drought stress in tomato seedlings were investigated. Growing patterns of tomato based on fresh weight of tomato seedlings were severely affected by chilling and drought stress. Fresh weight of tomato seedlings were reduced by 69.5% in chilling stress and 50.6% in drought stress compared to those in the unstressed control seedlings after 12 days of stress. The specific and gel activity of SOD and POD in the leaves, shoots, and roots of tomato seedlings were significantly increased by chilling and drought stress. Activation of SOD and POD activity by chilling stress were higher in the roots than those of drought stress. However, activation of SOD and POD activity by drought stress were higher in the leaves and shoots than those of chilling stress. The specific and gel activity of GR in the leaves, shoots, and roots of tomato seedlings were also significantly increased by chilling and drought stress. When the seedlings were treated with chilling or drought stress, one GR isozyme band (GR-3) was newly expressed in the leaves of tomato seedlings. The specific and gel activity of PPO was significantly increased in the roots and shoots of tomato seedlings by chilling and drought stress, respectively. However, the specific and gel activity of PPO in the leaves is no difference between stressed and controlled tomato seedlings.

목차 (Table of Contents)

• Abstract
• 서언
• 재료 및 방법
• 결과 및 고찰
• 적요
• 인용문헌

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