경쟁적인 고체상 면역분석법 : 생물발광 면역분석법과 효소 면역분석법의 비교 = Heterogeneous Competitive Immunoassay : Comparison between Bioluminescence Immunoassay and Enzyme Immunoassay

다국어 초록 (Multilingual Abstract)

A sensitive heterogeneous competitive immunoassay was developed for methamphetsunine (MA) by using two different labels. In immunoassay various labels can be selected as a signal generator. They are affected to a detection range and a limit of detection by detection system according to label used. Design of an assay are also one of the important factors. Here, same design was chosen with bioluminescence Immmunoassay (BLIA) and enzyme Immunoassay (EIA), in methamphetamine assay, and compared with both results. BLIA was performed with photoprotein, aequorin (AEQ) as a label and EIA was with alkaline phosphatase (AKP). AEQ is a biolummescent protein, which is originally isolated from the jellyfish Aequorea victoria. Photoproteins are attractive as labels in analytical applications because the bioluminescence signal has virtually no background. Thus, it allows for sensitive detection. Also, AKP has been known as a sensitive label as a single substrate enzyme. Optimal conditions of these assays were investigated to improve the sensitivity and lower the detection limit in each case.