Developmental Characteristics of SCNT Pig Embryos Knocked-out of Alpha-1,3-Galactosyltransferase Gene

목차 (Table of Contents)

• ABSTRACT
• INTRODUCTION
• MATERIALS AND METHODS
• Chemicals
• In Vitro Maturation of Oocytes
• Nuclear Transfer Procedure
• Collection of In Vivo Blastocysts
• TUNEL Assay for Apoptotic Cell Death
• Apoptosis Related Gene Expression
• Statistical Analysis
• RESULTS
• In Vitro Maturation of Porcine Oocytes
• In Vitro Development of SCNT Embryos
• Apoptosis Rate in mNT and GalT KO NT Blastocysts
• Analysis of Apoptosis Gene Expression in Blastocysts
• DISCUSSION
• REFERENCES

참고문헌 (Reference)

1 Qiao S, "p53, Bax and Bcl-2 expression, and apoptosis in gestational trophoblast of complete hydatidiform mole" 19 : 361-369, 1998

2 Joziasse DH, "Xenotransplantation: the importance of the Galalpha1,3Gal epitope in hyperacute vascular rejection" 1455 : 403-418, 1999

3 Xiao D, "Upregulation of Bax and Bcl-2 following prenatal cocaine exposure induces apoptosis in fetal rat brain" 5 : 295-302, 2008

4 Prather RS, "Transgenic swine for biomedicine and agriculture" 59 : 115-123, 2003

5 Lenton EA, "The endocrine environment of the human oocyte" 82 : 827-841, 1988

6 Galili U, "The alpha-gal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc-R) in xenotransplantation" 83 : 557-563, 2001

7 Dai Y, "Targeted disruption of the alpha 1,3-galactosyltransferase gene in cloned pigs" 20 : 251-255, 2002

8 Zhao J, "Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer" 81 : 525-530, 2009

9 Logan JS, "Prospects for xenotransplantation" 12 : 563-568, 2000

10 Nottle MB, "Production of homozygous α-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer" 14 : 339-344, 2007

11 Forsberg EJ, "Production of cloned cattle from in vitro systems" 67 : 327-333, 2002

12 Kolber-Simonds D, "Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations" 101 : 7335-7340, 2004

13 Phelps CJ, "Production of 1,3-galactosyltransferase-deficient pigs" 299 : 411-414, 2003

14 Hwang IS, "Osmolarity at early culture stage affects development and expression of apoptosis related genes (Bax-α and Bcl-xl) in pre-implantation porcine NT embryos" 75 : 464-471, 2008

15 Zakhartchenko V, "Nuclear transfer in cattle with non-transfected and transfected fetal or cloned transgenic fetal and postnatal fibroblasts" 60 : 362-369, 2001

16 Wang WH, "Morphologic evaluation and actin filament distribution in porcine embryos produced in vitro and in vivo" 60 : 1020-1028, 1999

17 Hara A, "Localization of Bax and Bcl-2 proteins, regulators of programmed cell death, in the human central nervous system" 429 : 249-253, 1996

18 Kolbe T, "Intracytoplasmic injection (ICSI) of in vivo or in vitro matured oocytes with fresh ejaculated or frozen-thawed epididymal spermatozoa and additional calcium ionophore activation in the pig" 52 : 671-682, 1999

19 Cooper DK, "Genetically engineered pigs" 342 : 682-683, 1993

20 Im GS, "Fragmentation and development of preimplantation porcine embryos derived by parthenogenetic activation and nuclear transfer" 71 : 159-165, 2005

21 Funahashi H, "Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro" 98 : 179-185, 1993

22 Fujimura T, "Effects of recloning on the efficiency of production of alpha 1,3 galactosyltransferase knockout pigs" 54 : 58-62, 2008

23 Viet Linh N, "Effects of cysteine during in vitro maturation of porcine oocytes under low oxygen tension on their subsequent in vitro fertilization and development" J Reprod Dev Epub ahead of print 2009

24 Hyo-Jin Moon, "Effects of FBS(Fetal Bovine Serum) and pFF(Porcine Follicular Fluid)on In Vitro Maturation and Development of PorcineParthenogenetic and Nuclear Transfer Embryos" 한국동물번식학회 33 (33): 85-91, 2009

25 Cheong HT, "Development of reconstituted pig embryos by nuclear transfer of cultured cumulus cells" 12 : 15-20, 2000

26 Iguma LT, "Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells" 4 : 55-66, 2005

27 박주희, "Comparison of Microtubule Distributions between Somatic Cell Nuclear Transfer and Parthenogenetic Porcine Embryos" 한국동물번식학회 33 (33): 13-18, 2009

28 Kobayashi M, "Blastocyst production by in vitro maturation and development of porcine oocytes in defined media following intracytoplasmic sperm injection" 15 : 93-102, 2007

29 Oltvai ZN, "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death" 74 : 609-619, 1993

30 Yin XM, "Bcl-2 gene family and the regulation of programmed cell death" 59 : 387-393, 1994

31 Levy RR, "Apoptosis in preimplantation mammalian embryo and genetics" 106 : 101-108, 2001

32 Hao Y, "Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro or by nuclear transfer" 69 : 501-507, 2003

33 Brenner CA, "Apoptosis and human embryo survival" Supplement 1 : 86-, 1997

34 Byrne AT, "Analysis of apoptosis in the preimplantation bovine embryo using TUNEL" 117 : 97-105, 1999