Electrochemical Property and Antibacterial Effect of Graphene Oxide Modified Zinc Oxide Particles

다국어 초록 (Multilingual Abstract)

This work introduces simple methods with low-cost and low time-consuming for synthesis of ZnO particles and preparation of ZnO/graphene oxide hybrids. Graphene oxide was prepared by modified Hummer method. The surface of ZnO particles were covered by amino group, due to 3-aminopropyl triethoxysilane (APTS) modified. Therefore, ZnO-APTS particles with amino groups can bond with hydroxyl and carboxyl easily. Fourier transform infrared (FT-IR), X-ray diffractometer (XRD), UV-visible spectrometer, field emission scanning electron microscopy (FE-SEM) and bio-atomic force microscope (Bio-AFM) revealed ZnO particles with novel shape were uniformly covered on the surface of graphene oxide sheets, and hybrids were stable and homogeneous in alcohol. Unique and interesting feature were exhibited in the ZnO/graphene oxide hybrids, which was not see in the individual structures. The electrochemical property of ZnO/graphene oxide hybrids modified Au-PCB electrode was demonstrated with apparent enhancement redox peak by cyclic voltammetry (CV). The highly efficient electrochemistry performance is helpful to manufacture a sensitive sensor and ideal material for application in super-capacitors. In addition, MIC method and flow cytometry were applied for verification of remarkable antibacterial properties of ZnO/graphene oxide hybrids comparison to pure ZnO particles, it meaningful for developing biomedicine and antibacterial
agents. Hence, applied ZnO/graphene oxide hybrids in more fields and explore its new function will be challenge for researchers, meanwhile, it has huge value for society.

목차 (Table of Contents)

• Contents
• Abstract
• List of Figures
• Chapter 1. Introduction 1
• Chapter 2. Experiment Section 5
• 2.1 Materials 5
• 2.2 Synthesis of ZnO particles 5
• 2.3 Synthesis of graphene oxide sheets 6
• 2.4 Synthesis ofZnO/ graphene oxide hybrids 7
• 2.5 Electrochemical measurement of graphene oxide and ZnO/graphene oxide hybrids 9
• 2.6 Antibacterial study 10
• 2.6.1 Determination of minimum inhibitory concentration 10
• 2.6.2 Live/Dead assay 10
• Chapter 3. Results and Discussions 11
• 3.1 Characterization of ZnO particles, graphene oxide sheets and ZnO/graphene oxide hybrids 11
• 3.1.1 Appearance and shape 11
• 3.1.2 Morphology and distribution 14
• 3.1.3 Absorption spectrum 16
• 3.1.4 X-ray diffraction (XRD) patterns 18
• 3.1.5 FT-IR spectra 20
• 3.1.6 Electrochemical property(CV) 22
• 3.2 Investigation of antibacterial properties 24
• 3.2.1 MIC of ZnO particles, graphene oxide sheets and ZnO/graphene oxide hybrids 24
• 3.2.2 Morphology of bacteria untreated and treated with ZnO particles, graphene oxide sheets and ZnO/graphene oxide hybrids, respectively. 26
• 3.2.3 Identification oflive and dead bacteria 29
• Chapter 4. Conclusions 31
• References 32
• Ackonwledgements 38