This study was performed to investigate the effects of pH on the degranulation of mesenteric and peritoneal mast cells induced by compound 48/80 in vivo and in vitro, and to determine the optimal dosage of compound 48/80 for the mast cell degranulatio...
This study was performed to investigate the effects of pH on the degranulation of mesenteric and peritoneal mast cells induced by compound 48/80 in vivo and in vitro, and to determine the optimal dosage of compound 48/80 for the mast cell degranulation in various pH.
Results were as following:
1. In vivo study using mesenteric mast cells : the effect of compound 48/80(1㎍/ml) on mast cells was markedly increased in pH 7.8.
2. In vitro study using peritoneal mast cells: the onset time of degranulation of mast cell induced by compound 48/80(0.33㎍/ml) was shorter in pH 7.2 and 7.8 than those in pH 5.5, 6.4 and 8.5.
Above results suggested that response of mast cell to compound 48/80 was rapidly degranulated in pH 7.2 and pH 7.8.