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      동형접합성 transgenic 미꾸라지 생산

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      https://www.riss.kr/link?id=E685900

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      다국어 초록 (Multilingual Abstract)

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      The transgene, pFV4CAT, containing CAT reporter gene regulated by carp β actin piomoter, was expressed in independent transgenic mud loach germ lines, determined by reverse transcriptase-PCR (RT-PCR) and enzyme-linked immunosorbant assay (ELISA). Expression of the transmitted transgene was found to be tissue-specific in F1 and F2 generations. Tissue specificity of the expression was dependent on each transgenic line with reproducible patterns Liver and spleen did express the transgene more frequently than other tissues tested, and muscle and heart revealed the higher amount of CAT than other tissues, while testes showed the lowest expression level. The highest level of CAT expression in muscle from a transgenic F1 line was corresponding to 68-fold compared to the basal levels of controls.

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      The transgene, pFV4CAT, containing CAT reporter gene regulated by carp β actin piomoter, was expressed in independent transgenic mud loach germ lines, determined by reverse transcriptase-PCR (RT-PCR) and enzyme-linked immunosorbant assay (ELISA). Exp...

      The transgene, pFV4CAT, containing CAT reporter gene regulated by carp β actin piomoter, was expressed in independent transgenic mud loach germ lines, determined by reverse transcriptase-PCR (RT-PCR) and enzyme-linked immunosorbant assay (ELISA). Expression of the transmitted transgene was found to be tissue-specific in F1 and F2 generations. Tissue specificity of the expression was dependent on each transgenic line with reproducible patterns Liver and spleen did express the transgene more frequently than other tissues tested, and muscle and heart revealed the higher amount of CAT than other tissues, while testes showed the lowest expression level. The highest level of CAT expression in muscle from a transgenic F1 line was corresponding to 68-fold compared to the basal levels of controls.

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      목차 (Table of Contents)

      • Ⅰ.Tissue-specifie expression of pFV4CAT in transgenic mud loach germ line
      • 1.Introduction
      • 2.Materials and methods
      • 3.Results
      • 4.Discussion
      • Ⅰ.Tissue-specifie expression of pFV4CAT in transgenic mud loach germ line
      • 1.Introduction
      • 2.Materials and methods
      • 3.Results
      • 4.Discussion
      • 5.References
      • 국문초록
      • Ⅱ.미꾸라지 expression vector의 활성도 조사
      • 1.서론
      • 2.실험재료 및 방법
      • 3.결과
      • 4.논의
      • 5.결론
      • 6.참고문헌
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