In this study, we characterized lung underlying mechanism in plasma and gDNA of asthmatics and to assess the effect of different mechanism compositions on the changes of lung function in F/U duration. We analyzed lung ROS, SOD, TAC, 8-OHdG and 5mC by ...
In this study, we characterized lung underlying mechanism in plasma and gDNA of asthmatics and to assess the effect of different mechanism compositions on the changes of lung function in F/U duration. We analyzed lung ROS, SOD, TAC, 8-OHdG and 5mC by using ELISA of the plasma and the gDNA paired sample of 60 asthmatics who were followed up for 1 year; 20 subjects with normal post-bronchodilator FEV1 (A), 20 patients with decreased FEV1 under 60% which showed a substantial increase lung function (B), and 20 patients with decreased FEV1 under 60% which did not increase during the follow up period (C). Compared with A, B groups, C groups had higher the relative increase of the SOD and TCA for F/U duration (p<0.01) in the plasma sample. However, the ROS was lower (p=0.014). Compared with A, B groups, C groups had a higher relative decrease of the 5mC for F/U duration in the gDNA sample (p=0.001). Significant alteration of oxidant, antioxidant, DNA damage and methylation in plasma and DNA were observed according to the refractory state of asthmatics. The asthma trajectory profiles may provide novel aspects for investigating pathophysiology and for developing a biomarker for refractory asthma. [funded by 2015-ER7402-00)]