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      Bacillus subtilis K-1에 의한 biopolymer의 생산과 levansucrase활성의 최적배양조건 = Optimal Culture Condition of the Biopolymer Production and Levansucrase Activity by Bacillus subtflls K-1 Strain

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      https://www.riss.kr/link?id=A75029763

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      다국어 초록 (Multilingual Abstract)

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      In order to study on the biopolymer production and the changes of its molecular composition, optimal culture condition for biopolymer production, levansucrase activity increase using identified strain and molecular structure and conformation of produced biopolymer were investigated. The mutants which was improved production ability of biopolymer and changed levansucrase activity were derived.
      The optimal culture conditions for bioplymer production and levansucrase activity increase were sucrose 6%, M.S.G. 2%, NaH₂P0₄·2H₂O 0.3%, MgSO₄·7H₂O 0.05%, biotin 0.1㎍/㎖ at pH 6.0, 37℃ for 48hours. Under this conditions, biopolymer production and levansucrase activity were about 25g/ℓ, 830 unit respectively.
      Among the several mutants which were gaind by UV light irradition, levansucrase activity of mutant M-4 was 3 fold higher and biopolymer production of mutant M-1 was 2 fold higher than wild type strain..
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      In order to study on the biopolymer production and the changes of its molecular composition, optimal culture condition for biopolymer production, levansucrase activity increase using identified strain and molecular structure and conformation of produc...

      In order to study on the biopolymer production and the changes of its molecular composition, optimal culture condition for biopolymer production, levansucrase activity increase using identified strain and molecular structure and conformation of produced biopolymer were investigated. The mutants which was improved production ability of biopolymer and changed levansucrase activity were derived.
      The optimal culture conditions for bioplymer production and levansucrase activity increase were sucrose 6%, M.S.G. 2%, NaH₂P0₄·2H₂O 0.3%, MgSO₄·7H₂O 0.05%, biotin 0.1㎍/㎖ at pH 6.0, 37℃ for 48hours. Under this conditions, biopolymer production and levansucrase activity were about 25g/ℓ, 830 unit respectively.
      Among the several mutants which were gaind by UV light irradition, levansucrase activity of mutant M-4 was 3 fold higher and biopolymer production of mutant M-1 was 2 fold higher than wild type strain..

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      목차 (Table of Contents)

      • 목차
      • Abstract
      • Ⅰ. 서론
      • Ⅱ. 재료 및 방법
      • Ⅲ. 결과 및 고찰
      • 목차
      • Abstract
      • Ⅰ. 서론
      • Ⅱ. 재료 및 방법
      • Ⅲ. 결과 및 고찰
      • Ⅳ. 요약
      • 참고문헌
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