인간 포미바이러스 인테그라제 돌연변이의 특성 = Characterization of Human Foamy Virus Integrase Mutant

다국어 초록 (Multilingual Abstract)

Human foamy virus (HFV) integrase mediates integration of viral c-DNA into cellular DNA. In this process, HFV integrase recognizes its own viral DNA specifically and catalyzes insertion of viral c-DNA. In order to study catalytic domains and residues, three deletion mutants and two point mutants of HFV integrase were constructed and analyzed with respect to enzymatic activities. The C-terminal deletion mutant showed decreased enzymatic activities while the N-terminal deletion mutant lost the activities completely, indicating that the N-terminal domain is more important than the C-terminal domain enzymatic reaction. The point mutants, in which an aspartic acid at the 164th position or a glutamic acid at the 200th position of The HFV integrase protein was changed to alanine, lost the enzymatic activities completely. However, they results suggest that the aspartic acid and glutamic acid at the respective 164th and 200th positions are catalytic residues for enzymatic reaction.

참고문헌 (Reference)

1 "mechanism of viral DNA cleavage and DNA strand transfer" 1991

2 "and electro- microscopy of a syncytial virus of lymphosarcomatous and apparently normal cattle" immu immu (immu immu): 1969

3 "a group of complex retroviruses" 1991

4 "The in protein of moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro" 1990

5 "Syncytium-forming agents isolated from domestic cats" 1969

6 "Role of the nonspecific DNA-binding region and helices within the core domain of retroviral integrase in selecting target DNA sites for integration" 276 : 2001

7 "Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus" Vincent -255, 1992

8 "Retroviral integrase functions as a multimer and can turn over catalytically" 1992

9 "Replication of retroviridae" Raven Press 1990

10 "Isolation of a virus from chimpanzee liver cell cultures inoculated with sera containing the agent of non-A" Prince (n-b hepatitis) : -11, 1984

11 "Integrative recombination of retroviral DNA" 1988

12 "Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex" 1993

13 "Genetics of retroviral integration" 26 : 527-, 1992

14 "Gene splicing by overlap extension Methods in Enzymology 217" 1993

15 "Endonucleolytic cleavages and DNA-joining activities of the integration protein of human foamy virus" 1993

16 "Complementation between HIV integrase proteins mutated in different domains" 1993

17 "Comparison of enzymatic activities of the HIV-1 and HFV integrases to their U5 LTR substrates" 47 : 612-, 1999

18 "Characterization of the human spuma Retrovirus integrase by site-directed nutagenesis and by swapping the zinc finger domain of HIV-1" 270 : 1995

19 "Characterization of human immunodeficiency virus type 1 integrase mutants expressed in Escherichia coli" 7 : -7, 1997

20 "Biochemical characterization of human foamy virus integrase" 48 : 2004

21 "An unusual virus from a human nasopharyngeal carcinoma" 1971

22 "Activities of human immunodeficiency virus integration protein in vitro specific cleavage and integration of HIV DNA" -88, 1991