부산 연안역의 진핵플랑크톤 종다양성에 대한 메타게놈 분석 연구 = Metagenomic Approach on the Eukaryotic Plankton Biodiversity in Coastal Water of Busan (Korea)

다국어 초록 (Multilingual Abstract)

The species composition of plankton is essential to understand the material and energy cycling within marine ecosystem. It also provides the useful information for understanding the properties of marine environments due to its sensitivity to the physicochemical characteristics and variability of water masses. In this study we adopted metagenomics to evaluate eukaryotic plankton species diversity from coastal waters off Busan. Characteristics of water masses at sampling sites is expected to be very complex due to the mixing of various water masses; Nakdong River runoff, Changjiang diluted water (CDW), South Sea coastal water, and Tsushima warm current. 18S rDNA clone libraries were constructed from surface waters at the three sites off Busan. Clone libraries revealed 94 unique phylotypes from 370 clones; Dinophyceae(42 phylotypes), Ciliophora(15 phylotypes), Bacillariophyta(7 phylotypes), Chlorophyta(2 phylotypes), Haptophyceae(1 phylotype), Metazoa(Arthropoda( 17 phylotypes), Chaetognatha(1 phylotypes), Cnidaria(2 phylotypes), Chordata(1 phylotype)), Rhizaria (Acantharea(2 phylotypes), Polycystinea(1 phylotype)), Telonemida(1 phylotype), Fungi(2 phylotypes). The difference in species diversity at the closely located three sites off Busan may be attributed to the various physicochemical properties of water masses at these sites by the mixture of water masses of various origins. Metagenomic study of species composition may provide useful information for understanding marine ecosystem of coastal waters with various physicochemical properties in the near feature.

참고문헌 (Reference)

1 최상, "한국해역의 식물풀랭크톤의 연구. 4. 동해, 남해 및 서해해역의 식물플랑크톤" 4 : 49-67, 1969

2 김형신, "한국 연안해역의 착편모조 분포" 한국양식학회 17 (17): 133-138, 2004

3 정익교, "한국 연안 초미세플랑크톤 생태: 1. 대한해협 서수도 해역" 13 : 101-107, 1998

4 심재형, "한국 동해 남부해역의 일차생산계. II. 식물플랑크톤 군집구조" 30 : 163-169, 1995

5 김일남, "하계 대한해협 저층냉수의 물리·화학적인 특성 및 기원" 한국해양연구원 26 (26): 595-606, 2004

6 문은영, "팔당호 유입부 경안천의 섬모충 플랑크톤 계절적 분포" 한국하천호수학회 41 (41): 11-18, 2008

7 심재형, "춘계 한국 동해 남부해역에서의 식물 수문학적 수역과 질산염약층의 수직양상" 24 : 15-28, 1989

8 최정민, "천수만의 표층 퇴적물 및 저서 유공충의 분포 특성과 퇴적환경" 한국고생물학회 26 (26): 107-128, 2010

9 김요혜, "제주도 남부해역의 부유성 섬모충류의 종 조성과 계절 변동" 한국해양학회 8 (8): 1-69, 2003

10 정혜진, "전북 새만금 남쪽 해역의 유해성 적조 발생연구2. 1999년도 여름-가을 종속영양성 와편모류와 섬모충류의 시각적 변화" 한국해양학회 7 (7): 5-147, 2002

11 권기영, "섬진강 하구역에서 염분경사에 따른 동식물플랑크톤의 현존량 및 종조성" 6 : 93-102, 2001

12 김보경, "분자 모니터링을 이용한 서낙동강과 남해 연안 플랑크톤 군집 분석" 한국해양학회 15 (15): 25-35, 2010

13 강소라, "북동중국해 내대륙붕 코아 퇴적물의 저서유공충 군집 특성과 고해양환경 변화" 대한지질학회 46 (46): 395-411, 2010

14 문성용, "남해 연안 동물플랑크톤 군집의 계절변동" 한국해양연구원 32 (32): 411-426, 2010

15 김영옥, "남해 거제도 장목만에서 부유성 섬모충의 시기별 분포 특성" 한국해양연구원 30 (30): 419-426, 2008

16 최철만, "낙동강하류의 식물플랑크톤상과 군집구조" 한국환경농학회 30 (30): 159-170, 2007

17 문창호, "낙동강 하구 환경특성 및 식물플랑크톤의 군집구조에 관한 연구" 26 : 144-154, 1991

18 Guillou, L., "Widespread occurrence and genetic diversity of marine parasitoids belonging to syndiniales (Alveolata)" 10 : 3349-3365, 2008

19 Duffy, J.E., "Why biodiversity is important to oceanography: potential roles of genetic, species, and trophic diversity in pelagic ecosystem processes" 311 : 179-189, 2006

20 Tsuda, A., "White-noise-like distribution of the oceanic copepod Neocalanus cristatus in the subarctic North Pacific" 97 : 39-46, 1993

21 López-García, P., "Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton" 409 : 603-607, 2001

22 Leppard, G.G., "The ultrastructural indicators of aquatic ecosystem health" 1 : 309-317, 1992

23 Palenik, B., "The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation" 104 : 7705-7710, 2007

24 McDonald, S. M., "The mixed lineage nature of nitrogen transport and assimilation in marine eukaryotic phytoplankton: a case study of Micromonas" 27 : 2268-2283, 2010

25 DeLong, E. F., "The microbial ocean from genomes to biomes" 459 : 200-206, 2009

26 Medlin, L. K., "The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions" 71 : 491-499, 1988

27 Huang, C., "The abundance cycle and influence factors on red tide phenomena of Noctiluca scintillans (Dinophyceae) in Dapeng Bay, the South China Sea" 19 : 303-318, 1997

28 Rush, D.B., "The Sorcerer II global ocean sampling expedition: northwest Atlantic through eastern tropical Pacific" 5 : e77-, 2007

29 Papillon, D., "Systematics of Chaetognatha under the light of molecular data, using duplicated ribosomal 18S DNA sequences" 38 : 621-634, 2006

30 Takano, Y., "Surface ultrastructure and molecular phylogenetices of four unarmored heterotrophic dinoflagellates, including the type species of the genus Gyrodinium (Dinophyceae)" 52 : 107-116, 2004

31 Pauly, D., "Primary production required to sustain global fisheries" 374 : 255-257, 1995

32 Gilg, I. C., "Phylogenetic affiliations of mesopelagic Acantharia and Antharian-like environmental 18S rDNA genes off the southern California coast" 161 : 197-211, 2010

33 Monier, A., "Phosphate transporters in marine phytoplankton and their viruses: cross-domain commonalities in viral-host gene exchanges" 14 : 162-176, 2012

34 Swofford, D. L., "PAUP: Phylogenetic Analysis Using Parsimony(and Other Methods)" Sinauer 2001

35 Moon-van der Staay, "Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity" 409 : 607-610, 2001

36 Louie, L. W., "Nutrient-regulated transcriptional responses in the brown tide-forming alga Aureococcus anophagefferens" 13 : 468-481, 2011

37 Montani, S., "Nutrient regeneration in coastal sea by Noctiluca scintillans, a red tide causing dinoflagellate" 6 : 224-228, 1998

38 Kim, E., "Newly identified and diverse plastid-bearing branch on the eukaryotic tree of life" 108 : 1496-1500, 2011

39 Not, F., "New insights into the diversity of marine picoeukaryotes" 4 : e7143-, 2009

40 Jeanmougin, F., "Multiple sequence alignment with Clustal X" 23 : 403-405, 1998

41 Agatha, S., "Morphologic and genetic variability in the marine planktonic ciliate Laboea strobila Lohmann, 1908 (Ciliophora, Oligotrichia), with notes on its ontogenesis" 51 : 267-281, 1908

42 Edvardsen, B., "Molecular systemtics of Haptophyta" 75 : 183-196, 2007

43 Hoppenrath, M., "Molecular phylogeny of ocelloid-bearing dinoflagellates (Warnowiaceae) as inferred from SSU and LSU rDNA sequences" 9 : 116-, 2009

44 Bowler, C., "Microbial oceanography in a sea of opportunity" 459 : 180-184, 2009

45 Simon, C., "Metagenomic analyses: past and future trends" 77 : 1153-1161, 2011

46 Collins, A. G., "Medusozoan phylogeny and character evolution clarified by new large and small subunit rDNA data and an assessment of the utility of phylogenetic mixture models" 55 : 97-115, 2006

47 Maddison, W.P., "MacClade: analysis of phylogeny and character evolution" Sinauer Associates 1991

48 Snoeyenbos-West, O. L. O., "Insights into the diversity of choreotrich and oligotrich ciliates(Class: Spirotrichea) based on genealogical analyses of multiple loci" 52 : 1901-1913, 2002

49 Lee, S.-R., "Effective molecular examination of eukaryotic plankton species diversity in environmental seawater using environmental PCR, PCR-RFLP and sequencing" 22 : 699-707, 2010

50 Itaki, T., "Depth-related radiolarian assemblage in the water-column and surface sediments of the Japan Sea" 47 : 253-270, 2003

51 Matheny, P.B., "Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi)" 43 : 430-451, 2007

52 Romari, K., "Composition and temporal variability of picoeukaryote communities at a coastal site of the English Channel from 18S rDNA sequences" 49 : 784-798, 2004

53 Bianchi, F., "Can plankton communities be considered as bio-indicators of water quality in the laggon of Venice?" 46 : 964-971, 2003

54 Falkowski, P. G., "Biogeochemical controls and feedbacks on ocean primary production" 10 : 200-206, 1998

55 Jones, E. B. G., "Are there more marine fungi to be described?" 54 : 343-354, 2011

56 Medlin, L. K., "A molecular clock for Coccolithophores and implications for selectivity of phytoplankton extinctions across the K/T boundary" 67 : 69-86, 2008

57 오현주, "2004년 하계 남해안 해황과 식물플랑크톤의 분포 특성" 한국지리정보학회 10 (10): 40-48, 2007