<P><B>Abstract</B></P><P>Hydrogen (H<SUB>2</SUB>) production from glucose by dark fermentation suffers from the low yield. As a solution to this problem, co‐production of H<SUB>2</SUB> and et...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A107484232
2016
-
SCOPUS,SCIE
학술저널
249-256(8쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P><B>Abstract</B></P><P>Hydrogen (H<SUB>2</SUB>) production from glucose by dark fermentation suffers from the low yield. As a solution to this problem, co‐production of H<SUB>2</SUB> and et...
<P><B>Abstract</B></P><P>Hydrogen (H<SUB>2</SUB>) production from glucose by dark fermentation suffers from the low yield. As a solution to this problem, co‐production of H<SUB>2</SUB> and ethanol, both of which are good biofuels, has been suggested. To this end, using <I>Escherichia coli</I>, activation of pentose phosphate (PP) pathway, which can generate more NADPH than the Embden‐Meyhof‐Parnas (EMP) pathway, was attempted. Overexpression of two key enzymes in the branch nodes of the glycolytic pathway, Zwf and Gnd, significantly improved the co‐production of H<SUB>2</SUB> and ethanol with concomitant reduction of pyruvate secretion. Gene expression analysis and metabolic flux analysis (MFA) showed that, upon overexpression of Zwf and Gnd, glucose assimilation through the PP pathway, compared with that of the EMP or Entner‐Doudoroff (ED) pathway, was greatly enhanced. The maximum co‐production yields were 1.32 mol H<SUB>2</SUB> mol<SUP>−1</SUP> glucose and 1.38 mol ethanol mol<SUP>−1</SUP> glucose, respectively. It is noteworthy that the glycolysis and the amount of NAD(P)H formed under anaerobic conditions could be altered by modifying (the activity of) several key enzymes. Our strategy could be applied for the development of industrial strains for biological production of reduced chemicals and biofuels which suffers from lack of reduced co‐factors.</P>
Minimal genome: Worthwhile or worthless efforts toward being smaller?