구기자 투여가 나일틸라피아, Oreochromis niloticus 의 Edwardsiella tarda 백신 처리에 미치는 효과 = The dietary supplementing effects of Kugija , Lycium chinense , on immune responses of Nile tilapia , Oreochromis niloticus , to Edwardsie0a tarda

다국어 초록 (Multilingual Abstract)

To study the supplementaing effects of kugija, Lycium chinense, in commercial diet on the immune response of nile tilapia, Oreochromis niloticus, nonspecific immune responses were investigated. The activities of complement and lysozyme were higher in sera of the fish fed diet with kugija than control diet. The effects of kugija on vaccination of fish to Edwardsiella tarda were compared in three different vaccination methods after feeding kugija for ten weeks. Intraperitoneal injection and bath administration with formalin killed cells (FKC) and bath with extracellular products (ECP) after injection of FKC showed differences in immune responses of vaccinated fish. Bath administration with ECP after injection of FKC was more stimulated than any other methods in each of nonspecific and specific responses such as the activities of complement and lysozyme, antibody production and rosette forming cells. Moreover, cumulative mortality was significantly lower in the fish vaccinated with combination FKC and ECP after injection challenge with live E. tarda.