Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus

다국어 초록 (Multilingual Abstract)

Infectious bronchitis virus (IBV) poses a severe threat to thepoultry industry and causes heavy economic losses worldwide.
Vaccination is the most effective method of preventing infectionand controlling the spread of IBV, but currently availableinactivated and attenuated virus vaccines have somedisadvantages. We developed a chimeric virus-like particle(VLP)-based candidate vaccine for IBV protection. The chimericVLP was composed of matrix 1 protein from avian influenzaH5N1 virus and a fusion protein neuraminidase (NA)/spike 1 (S1)that was generated by fusing IBV S1 protein to the cytoplasmicand transmembrane domains of NA protein of avian influenzaH5N1 virus. The chimeric VLPs elicited significantly higherS1-specific antibody responses in intramuscularly immunizedmice and chickens than inactivated IBV viruses. Furthermore,the chimeric VLPs induced significantly higher neutralizationantibody levels than inactivated H120 virus in SPF chickens.
Finally, the chimeric VLPs induced significantly higher IL-4production in mice. These results demonstrate that chimericVLPs have the potential for use in vaccines against IBV infection.

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