Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice

다국어 초록 (Multilingual Abstract)

Activation of the innate immune system requires recognitionof pathogen-associated molecular patterns, such as NOD-likereceptors. The NOD-like receptor protein 3 (NLRP3)inflammasome is involved in induction of the proinflammatorycytokine, IL-1β, and subsequent inflammatoryresponses. NLRP3 inflammasome plays important roles in theinflammatory and innate immune responses associated withautoimmune/inflammatory syndrome. However, analysis of thetissue distribution and expression profiles in BALB/c mice is stillincomplete. In this study, we investigated the tissue distributionand expression pattern of NLRP3 in BALB/c mice to furtherelucidate its function in innate immunity in this commonly usedlaboratory animal model. NLRP3 mRNA expression levels andtissue distribution of the protein were investigated by real-timequantitative PCR and immunohistochemical analyses,respectively. NLRP3 mRNA expression was higher in thekidney and inguinal lymph nodes than in other tissues.
Cytoplasmic expression of NLRP3 was detected in the epithelialreticular cells of the spleen and thymus, lymphocytes in theinguinal lymph nodes, cardiac muscle cells, cerebral cortexneurons, alveolar macrophages, renal tubule cells and liversinusoidal endothelial cells. The results of this study will assistinvestigators in interpreting site-specific functions and roles ofNLRP3 in inflammatory responses.

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