In vitro propagation of a useful tropical bamboo, Thyrsostachys siamensis Gamble, through shoot-derived callus

다국어 초록 (Multilingual Abstract)

We established a protocol for mass propagation of Thyrsostachys siamensis Gamble in vitro culture via callus formation.
Single node cuttings were placed in Murashige and Skoog (MS) medium with benzyl adenine (BA) concentrations rangingfrom 0 to 44.40 μM. Although multiple shoots formed in all BA treatments, optimum shoot formation occurred at 11.10 μMBA. The MS medium for optimum callus induction from the multiple shoots contained 11.3 μM of 2,4-dichlorophenoxyaceticacid (2,4-D), 4.65 μM kinetin, and 1.96 μM indole-3-yl-butyric acid (IBA). The highest number of shoots regenerating fromthe callus was obtained in MS medium containing 11.1 μM N 6 -BA and 3.43 μM IBA. Shoot clusters of 3–5 shoots wererooted on MS medium supplemented with 26.85 μM 1-naphthaleneacetic acid for 3 weeks, and were then transferred ontoMS medium without plant growth regulators for 1 week. This protocol can be used for gene manipulation in a breedingprogram, germplasm preservation, and mass propagation of the bamboo.

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