Thermostable DNA ligase is an essential enzyme for the ligase chain reaction(LCR), a method of detection of a single point mutation. Firstly reported thermostable DNA ligase gene was Tth lig gene of Thermus thermophilus HB8 in 1991. We amplified a new...
Thermostable DNA ligase is an essential enzyme for the ligase chain reaction(LCR), a method of detection of a single point mutation. Firstly reported thermostable DNA ligase gene was Tth lig gene of Thermus thermophilus HB8 in 1991. We amplified a new thermostable DNA ligase gene of Thermous caldophilus GK24 using the method of PCR. Used degenerate primers DLI and DLT were designed based on Tth DNA ligase gene. This amplified Tca DNA ligase gene was cloned and expressed. For DNA subcloning and sequencing, restriction enzyme sites of the gene were analyzed.