Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against Plant Pathogenic Bacteria Ralstonia solanacearum

다국어 초록 (Multilingual Abstract)

An antagonistic bacterial strain KLF01 was isolated from rhizosphere of tomato and identified to be Lactobacillus sp. by biochemical and genetic analysis. This strain showed antagonism against the used plant pathogenic bacteria like Ralstonia solanacearum, (bacterial wilt), Xanthomonas axonopodis pv. citri, (Citrus canker), Xanthomonas campestris pv. vesicatoria (Bacterial spot), Eriwinia pyrifoliae (Shoot-blight) and Eriwinia carotovora subsp. carotovora group (Potato scab) through agar well diffusion method. In planta test done by drench application of strain KLF01 (4×10? cfu/㎖) into the experimental plot containing tomato (Solanum lycopersicum L.) cultivar 'Lokkusanmaru' and red pepper (Capsicum annuum L.) cultivar 'Buja' plants, in pot test post-inoculated with the plant pathogenic bacteria, R. solanacearum significantly reduced the disease severity, compared to the non-treated plants.

참고문헌 (Reference)

1 성필제, "풋마름병균, Ralstonia solanacearum의 길항세균 SKU-78 균주의 분리 동정 및 특성" 한국응용생명화학회 48 (48): 48-52, 2005

2 석정기, "토양에서 분리한 Paenibacillus spp. KPB3의 Ralstonia solanacearum에 의한 세균성 풋마름병 억제 효과" 한국농약과학회 10 (10): 313-319, 2006

3 Wen-Hsin Lin., "Viable counts, characteristic evaluation for commercial lactic acid bacteria products" 23 : 74-81, 2006

4 Sharpe, M. E, "The genus Lactobacillus in: The prokaryotes" The prokaryotes 1653-1679, 1981

5 Wood, B. J. B, "The genera of lactic acid bacteria" Blackie Academic & Professional 1955

6 Hammes, W.P, "The genera Lactobacillus and Carnobcterium. In the prokaryotes. A handbook on the biology of bacteria: ecoophysiology, isolation, identification, applications"

7 Dalal, N. R, "Studies on grading and pre-packaging of some bacterial wilt resistant brinjal(Solanum melongena L.)" 9 : 223-226, 1999

8 Stirling,A.C., "Sources of the lactic acid bacteria occurring in silage" 26 : 86-90, 1963

9 Lemessa,F, "Screening rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia" 42 : 336-344, 2007

10 Suzuki,T., "Phylogeny of spore-forming LAB based on 16S rRNA gene sequences" 115 : 13-17, 1994

11 Sambrook,J, "Molecular Cloning: A laboratory manual" Cold harbour laboratory press 2001

12 Mundt, J. O, "Lactobacilli on plants" 16 : 1326-1330, 1968

13 Bernal,G., "Isolation and partial purification of a metabolite from a mutant strain of Bacillus sp.with antibiotic activity against plant pathogenic agents." 2002

14 Chen, Y.-S, "Isolation and identification of lactic acid bacteria from soil using an enrichment procedure." 40 : 195-200, 2005

15 Benkerroum,N., "Inhibitory action of nisin against Listeria monocytogenes" 71 : 3237-3245, 1988

16 Piard J.C., "Inhibiting factors produced by lactic acid bacteria: oxygen metabolites and end-products from catabolism" 71 : 525-541, 1991

17 Perry, S.F, "Freeze drying and cryopreservation of bacteria. In: methods in molecular biology" 21-30, 1995

18 El Abdyal, M. S, "Effects of culture conditions on the antimicrobial activities of UV-mutants of Streptomyces corchorusii and S.spiroverticillatus against bean and banana wilt pathogens." 151 : 201-211, 1996

19 Vincent,V.M., "Effect of a soil amendment on the survival of Ralstonia solanacearum in different soils" 88 : 300-305, 1998

20 Katayama.K, "Ecology and protection of bacterial wilt of potato 2. Some control methods and their integration" 15 : 29-57, 1987

21 Molina, L, "Construction assays" 64 : 2072-2078, 1998

22 Hamad S.H., "Characterization of the bacterial flora of sudanese sorghum flour and sorghum sourdough." 83 : 764-770, 1997

23 HAN, KYOUNG-SIK, "Characterization and Purification of Acidocin 1B, a Bacteriocin Producedby Lactobacillus acidophilus GP1B" 한국미생물·생명공학회 17 (17): 774-783, 2007

24 Stiles,E.M, "Biopreservation by lactic acid bacteria." 70 : 331-345, 1996

25 Hayward, A. C, "Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum" 29 : 65-87, 1991

26 Dong,C., "Biological control of tomato bacterial wilt with avirulent bacteriocinogenic strain of Ralstonia solanacearum." 20 : 1-4, 1999

27 Kang,Y., "Biological control of bacterial wilt of tomato by extracellular protein defective mutants of Pseudomonas solanacearum" 22 : 287-288, 1995

28 Altschul, S.F, "Basic local alignment search tool" 215 : 403-410, 1990

29 Seuk Hyun Ko, "Bacteriocin production by Lactococcus lactis KCA2386 isolated from white kimchi" 9 : 263-269, 2000

30 Ouwehand, A.C, "Antimicrobial components from lactic acid bacteria. in lactic acid bacteria microbiology and functional aspects ed. Salminen, S. and von Wright, A" Marcel Dekker 139-159, 1998

31 Laitila, A, "Antifungal activities of two Lactobacillus plantarum strains against fusarium moulds in vitro and in malting of barley" 93 : 556-576, 2002

32 Ronel Visser, "Antagonism of lactic acid bacteria against phytopathogenic bacteria" 52 : 552-555, 1986

33 Weisburg,W.G., "16S ribosomal DNA amplification for phylogenetic study" 173 : 697-703, 1991