Stenotrophomonas maltophilia의 획득내성유전자 분석

다국어 초록 (Multilingual Abstract)

Background: S. maltophilia is a non-fermentative, Gram-negative bacillus, increasingly identified as a nosocomial pathogen in immune compromised patients, Trimethoprim/sulfamethoxazole is one of the most potent agents for treating S. maltophilia infection. But, resistance to trimethoprim/sulfamethoxazole is increasing. In this study, we have investigated the relation between the incidence of trimethoprim/ sulfamethoxazole resistance and the presence of sul1, class1 integron, and between the incidence of ciprofloxacin resistance and the presence of Smqnr gene.

Materials and methods: Seventy-six S. maltophilia isolates were collected at the Chungnam National university Hospital, Chungbuk National university Hospital, Eul-Ji university Hospital during April-November 2007. Antibiotics susceptibility were tested by Disk diffusion method. PCR and DNA sequencing were conducted for the detection of sul1 gene, class1 integron and Smqnr gene.

Results: Eleven of 76 isolates were resistant for Trimethoprim/ sulfamethoxazole. Harbouring rate of sul1 gene, class1 integron were about 81.8%, 72.7% in resistant isolates and about 16.8%, 10.9% in susceptible isolates, respectively. Twenty-nine of 76 isolates were resistant for ciprofloxacin. 10/29(34.5%) of ciprofloxacin resistant S. maltophilia were positive for Smqnr. 19/44(43.2%) of ciprofloxacin susceptible S. maltophilia were positive for Smqnr.

Conclusion: S. maltophilia harbouring Su1 gene, class 1 integron were higher resistant for trimethoprim/ sulfamethoxazole (P-value:<0.01). S. maltophilia harbouring Smqnr were not higher resistant for ciprofloxacin (P-value=0.41).

목차 (Table of Contents)

• Ⅰ. 서론 1
• Ⅱ. 재료 및 방법 3
• Ⅲ. 결과 7
• Ⅳ. 고찰 10
• Ⅴ. 결론 13
• 참고문헌 15
• ABSTRACT 19