산민들레 종자의 발아특성 연구

다국어 초록 (Multilingual Abstract)

Background: Many Taraxacum spp. plants are widely used in medicine, but some of them have propagation problems, such as strong dormancy and poor germination rates. This study investigated the effects of temperature, gibberellic acid (GA3), and potassium nitrate (KNO3) on seed germination in Taraxacum ohwianum.
Methods and Results: The seeds (NIBRGR0000135524) were exalbuminous, and their length and width were 4.54 ± 0.032㎜ and 0.97 ± 0.029㎜, respectively. Various germination temperatures were tested (15, 20, 25, and 30℃), the optimum temperature for germination was found to be 20℃ (31.3%). High temperature (30℃) resulted in non-typical seedlings (thickened and crumpled cotyledons, with a restricted root system). To increase the germination capacity, GA3 (200, 500, and 1,000 ㎎/ℓ ) or KNO3 (20, 50, 100, 200, and 500 ㎎/ℓ ) solutions were used as pre-soaking solutions instead of water. The GA3 treatments increased the germination percentage and rate, but germination percentage was higher with the KNO3 treatments. Under the 50 - 200 ㎎/ℓ KNO3 treatments, the germination percentage exceeded 80% after 12 days, and 50 ㎎/ℓ KNO3 was notably effective (91.2% after 15 days).
Conclusions: T. ohwianum seeds showed improved germination at low temperatures. The best method for germination was presoaking in 50 ㎎/ℓ KNO3 for 24 h, in the dark at 4℃, and then incubating the germinated seed at 20℃ for 15 days.

목차 (Table of Contents)

• 서 언
• 재료 및 방법
• 1. 실험재료
• 2. 종자의 형태학적 특성 및 활력검증
• 3. 종자 발아 실험
• 4. 통계처리
• 결과 및 고찰
• 1. 종자의 형태적 특성 및 활력
• 2. 온도조건에 의한 발아특성
• 3. 지베렐린에 의한 발아촉진 효과
• 4. 무기염류에 의한 발아촉진 효과
• REFERENCES

참고문헌 (Reference)

1 이수광, "종자저장온도 및 전처리가 야생 하늘타리와 노랑하늘타리의 종자발아 및 유묘 생육특성에 미치는 영향" 한국약용작물학회 22 (22): 53-59, 2014

2 송상철, "제주지역 상동나무의 열매와 종자발아 특성" 한국약용작물학회 23 (23): 8-12, 2015

3 김윤하, "식물생장조절물질 처리가 서양민들레 종자 발아에 미치는 영향" 한국잔디학회 2 (2): 152-158, 2013

4 김도현, "생장조절제와 프라이밍 처리에 의한 지치종자의 발아특성과 단백질 발현 양상에 관한 연구" 한국약용작물학회 22 (22): 435-441, 2014

5 김도현, "생장조절제와 프라이밍 처리에 의한 우슬종자의 발아특성과 단백질 발현 양상에 관한연구" 한국약용작물학회 23 (23): 13-19, 2015

6 김태완, "민들레 뿌리로부터 Pancreatic lipase 저해 물질의 분리" 한국식품저장유통학회 18 (18): 53-58, 2011

7 강진호, "국내 학술지에 발표된 약용작물 종자처리의 실용성 분석" 한국약용작물학회 12 (12): 328-341, 2004

8 최경희, "국내 자생 민들레 5종의 항산화 활성 및 Nitric Oxide 생성억제 활성" 한국약용작물학회 21 (21): 191-196, 2013

9 최충호, "갈매보리수나무 종자의 온도 및 여러 가지 전처리에 따른 발아반응" 한국자원식물학회 25 (25): 132-141, 2012

10 Yoon ST, "The technology for increasing seed viability by priming treatment and plant growth regulators in medicinal plants" 11 : 85-95, 1999

11 Raisi A, "The study effects of stratificaion, temperature and potassium nitrate on seed dormancy breaking Ferula assa-foetida" 21 : 379-383, 2013

12 Phartyal SS, "The influences of temperatures on seed germination rate in Himalayan elm(Ulmus wallichiana)" 31 : 83-93, 2003

13 Coolbear P, "The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds" 35 : 1609-1617, 1984

14 Ahn YH, "The comparisons of germination characteristic in native Taraxacum spp. and Taraxacum officinale Seeds" 14 : 199-204, 2000

15 Ministry of Food and Drug Safety, "The Korean herbal pharmacopeia" Ministry of Food and Drug Safety 418-418, 2016

16 Schütz K, "Taraxacum : A review on its phytochemical and pharmacological profile" 107 : 313-323, 2006

17 Kang BH, "Survey on wild edible plant resources in Korea and its germination characteristics" 42 : 236-246, 1997

18 National Institute of Biological Resources, "Species Korea" National Institute of Biological Resources

19 Bewley JD, "Seeds: Physiology of development and germination" Plenum Press 160-171, 1994

20 Gavassi MA, "Seed germination in tomato : A focus on interaction between phytochromes and gibberellins or abscisic acid" 5 : 2163-2169, 2014

21 National Institute of Biological Resources, "National list of species of Korea"

22 International Seed Testing Association, "International rules for seed testing. Chapter 6: The tetrazolium test" International Seed Testing Association 1-25, 2014

23 Sun HY, "In vitro antibacterial activities of antibiotics and traditional Chinese medicinal herb extracts on Escherichia coli and Staphylococcus aureus" 26 : 298-302, 2014

24 Washitani I., "Germination-rate dependency on temperature of Geranium carilinianum seeds" 36 : 330-337, 1985

25 Park JE, "Effect of several priming treatment on seed germination of Taraxacum coreanum Nakai" 32 (32): 208-208, 2014

26 Ahn SY, "Cultivation of wild vegetables"

27 정현환, "Chilling Requirements for Dormancy Breaking and Flowering of Adonis amurensis Regel et Radde" 한국원예학회 50 (50): 502-508, 2009

28 Mok SY, "Aldose reductase inhibitory activity of Taraxacum ohwianum" 23 (23): 88-88, 2010

29 Levitt J, "A method of increasing the rate of seed germination of Taraxacum kok-saghyz" 18 : 288-293, 1943