Fabrication of high-aspect-ratio polymeric nanoneedle arrays via UV nanoimprint lithography for guided cell culture and differentiation = 세포 배양 및 분화 유도를 위한 UV 나노임프린트 리소그래피 이용한 고종횡비의 고분자 나노니들 제조

목차 (Table of Contents)

• ABSTRACT
• 국문 초록
• TABLE OF CONTENTS
• LIST OF TABLES
• LIST OF FIGURES
• ABBREVIATION
• CHAPTER 1. Introduction
• 1.1 Influence of Biophysical Cues at the Biointerfaces: Understanding Cellular Responses to Surface Properties
• 1.2 Nanogeometries for Guided Cell Culture
• 1.2.1 Regulation of cellular behavior by various nanotopographies
• 1.2.2 High-aspect-ratio nanoneedle structures for interfacing and influencing cells
• 1.3 Nanoimprint Lithography (NIL) Process for Fabricating High-aspect-ratio Nanostructures
• 1.4 Objectives of This Study
• CHAPTER 2. Experimental Section
• 2.1 Materials
• 2.2 Fabrication of PUA NNs
• 2.3 Scanning electron microscopy (SEM) of PUA NNs Arrays and Cell-Attached PUA NNs
• 2.4 SEM Nanoindentation
• 2.5 Cell Culture
• 2.6 Cell Proliferation and Viability Test
• 2.7 Immunocytochemistry
• 2.8 Live Cell Imaging
• 2.9 Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
• 2.10 Statistical Analysis
• CHAPTER 3. Results and Discussion
• 3.1 Fabrication of PUA Flat/NNs with Varying Stiffness
• 3.2 Enhanced Cell Migration of NIH 3T3 Cells on PUA NNs
• 3.3 Guided Cell Alignment of PC12 Cells on PUA NNs
• 3.4 Enhanced Neuronal Differentiation of ReN Cells on PUA NNs
• CHAPTER 4. Conclusion
• BIBLIOGRAPHY