황금의 진통 및 항염증효과에 대한 연구 = Analgesic and anti-inflammatory effect of Scutellaria baicalensis

다국어 초록 (Multilingual Abstract)

Backgrounds: Scutellaria baicalensis has been used as a medicinal plant to treat various disease conditions accompanying inflammatory response and oxidative stress.
Objectives: The aim of this study is to evaluate the effects of Scutellaria baicalensis against inflammatory, pain and edema.
Methods: In vitro, the effects of Scutellaria baicalensis against lipopolysaccharide-induced inflammation were investigated in mouse BV2 microglial cells. In vivo, the effects of Scutellaria baicalensis on acetic acid-induced writhing response, carrageenan-induced edema and the plantar test (nociceptive thermal stimulation) were investigated using rats and mice.
Results: The present results showed that pre-treatment with the aqueous extract of Scutellaria baicalensis suppressed the lipopolysaccharide-stimulated cyclooxygenase-2 expressions in mouse BV2 microglial cells. The aqueous extract of Scutellaria baicalensis inhibited acetic acid-induced abdominal pain in mice and also reduced thermal pain in rats. However, no significant inhibition on carrageenan-induced paw edema in rats.
Conclusions: The present study showed that Scutellaria baicalensis possesses anti-inflammatory and analgesic effects.

국문 초록 (Abstract)

배경 : 황금은 淸熱燥濕藥으로서 통증과 산화스트레스를 수반하는 염증질환에 임상적으로 다양하게 응용되고 있다.
목적 : 황금추출액의 항염증 및 통증, 부종, 열자극에 대한 효과를 알아보고자 한다.
방법 : In vitro 실험으로 mouse의 BV2 미세아교세포에 lipopolysaccaride가 유발한 염증에 대해 황금 추출액이 끼치는 효과를 연구하였다. 또한 in vivo 실험으로서, carrageenan으로 유발한 부종, 초산(acetic acid)로 유발된 복부 동통, 열을 이용한 통각과민에 대하여 황금추출액이 어떤 영향을 미치는지 알아보았다.
결과 : 황금 추출액은 mouse BV2 미세아교세포의 lipopolysaccharide가 유발시킨 cyclooxigenase-2의 합성을 억제하는 반응을 보였다. 또한, mouse에서 초산으로 유발한 복부 동통을 감소시켰으며, rat에서 열로 유발한 통각과민 실험에서도 유의미한 억제효과가 나타났다. 그러나, rat에서 carrageenan으로 유발한 부종실험에서는 유의미한 억제효과가 나타나지 않았다.
결론 : 황금은 주목할 만한 항염증 및 진통효과를 가지고 있는 것으로 사료된다.

목차 (Table of Contents)

• Ⅰ. Introduction = 1
• Ⅱ. Materials and methods = 4
• 1. Preparation of the aqueous extract of Scutellaria baicalensis = 4
• 2. In vitro experiments = 4
• 1) Reagents = 4
• 2) Cell culture = 4
• 3) MTT cytotoxicity assay = 5
• 4) Western blot analysis = 6
• 3. In vivo experiments = 7
• 1) Animals and treatments = 7
• 2) Acetic acid-induced writhing response in mice = 8
• 3) Carrageenan-induced edema in rats = 8
• 4) Plantar test (Hargreaves’s method) = 10
• 4. Data analysis = 11
• Ⅲ. Results = 12
• 1. Cell viability of Scutellaria baicalensis on BV2 microglia cells = 12
• 2. Effect of Scutellaria baicalensis on protein expression of COX-1 and COX-2 = 15
• 3. Effect of Scutellaria baicalensis on acetic acid-induced writhing response = 18
• 4. Effect of Scutellaria baicalensis on the volume of carrageenan-induced paw edema = 20
• 5. Effect of Scutellaria baicalensis on the plantar test (nociceptive thermal stimulation) = 24
• Ⅳ. Discussion = 27
• Ⅴ. References = 31
• ABSTRACT = 37