비교 유전체 보합법에 의한 두경부 편평상피세포암 세포주의 염색체 이상 분석 = Analysis of Chromosomal Aberrations in Head and Neck Squamous Cell Carcinoma Cell Lines using CGH

국문 초록 (Abstract)

최근 종양에서 흔히 보이는 염색체 이상에 대한 연구가 주목받고 있으며, 이러한 연구는 암의 진단법 개발과 예후 결정에 중요하다. 비교 유전체 보합법(comparative genomic hybridization, CGH)은 암 조직의 모든 염색체 상에서의 염색체 변화를 한 번에 알 수 있게 해 주는 유용한 방법이다. 이 연구에서는 CGH를 이용하여 두경부 편평상피세포암세포주 SNU-1041, SNU-1066, SNU-1076, PCI-1, PCI-13, PCI-50의 염색체 이상을 분석하였으며, 이를 바탕으로 두경부 편평상피세포암의 발생 및 진행과 관련된 염색체 이상을 밝히고자 하였다. 그 결과, 여섯 개의 세포주 모두에서 증가한 염색체 부위는 9q32-q34, 16q22-q24, 20q11.2-qter이었다. 염색체 3p10-p14의 감소가 다섯 개의 세포주에서 관찰되었으며, 3p15-p23, 4q22-q27, 4q31.3-qter, 18q21-q23의 감소가 네 개의 세포주에서 관찰되었다.
두경부 편평상피세포암 세포주의 염색체 이상에 대한 연구 결과는 암발생과 관련된 상세한 유전적 변화들을 이해하는 기본적인 단계가 될 것으로 판단된다.

다국어 초록 (Multilingual Abstract)

The knowledge about chromosomal aberrations manifestated in cancer has been spotlighted recently, and these studies are important for the development of cancer diagnosis and evaluation of prognosis.
Purpose of this study was examine the chromosomal aberrations which were related in the development and
progression of HNSCC. In this study, chromosomal aberrationsin HNSCC cell line, SNU-1041, SNU-1066, SNU-1076, PCI-1, PCI-13, and PCI-50 were analyzed using CGH.
As a results, gains on 9q32-q34, 16q22-q24, and 20q11.2-qter were observed in all six cell lines. Loss on 3p10-p14 was analyzed in five cell lines. Also, losses on 3p15-p23, 4q22-q27, 4q31.3-qter, and 18q21-q23 were observed in four cell lines.
These data about the patterns of chromosomal aberrations in HNSCC cell lines would be a basic step for
understanding more detailed genetic events in the carcinogenesis.

참고문헌 (Reference)

1 "Three discrete regions of deletion at 3p in headand neck cancers. Cancer Res 53" 5775-5779, 1993.

2 "The netrin-1 receptors UNC5H are putative tumor suppressors controlling cell death commitment" 100 : 4173-4178, 2003.

3 "The molecular genetics of cancer. Science 235" 305-311, 1991.

4 "Targeting head and neck cancer by GM-CSFmediatedgene therapy in vitro." 19 : 5335-5359, 1999.

5 "Significant involvementof chromosome 13q deletions in progression oflarynx cancer detected by comparative genomic hybridization." 35 : 407-413, 2005.

6 "REL proto-oncogene is frequently amplified in extranodal diffuse large cell lymphoma" 87 : 25-29, 1996.

7 "Potential role of caspase-3 and -9 in arsenic trioxidemediatedapoptosis in PCI-1 head and neck cnacer cells." 18 : 249-255, 2001.

8 "Optimizing comparative genomic hybridizationfor analysis of DNA sequence copy number changes insolid tumors. Genes Chromosome Cancer 10" 231-243, 1994.

9 "Loss of 18q predicts poor survivalof patients with squamous cell carcinoma of the head andneck." 21 : 333-339, 1998.

10 "Inhibitory effect of p27KIP1 genetransfer on head and neck squamous cell carcinoma celllines." 25 : 44-49, 2003.

11 "Induction chemotherapy followed by concomitant chemoradiotherapy for advanced head and neck cancer: Impact on the natural history of the disease" 13 : 876-880, 1995.

12 "Identification of new minimally lost regions on 18q in head and neck squamous cell carcinoma." 60 : 3397-3403, 2000.

13 "Human cytotoxic T-celllines with restricted specificity for squamous cell carcinomaof the head and neck. Cancer Res 53" 1461-1468, 1993.

14 "Genomic alterations associated with malignancyin head and neck cancer. Head Neck 20" 145-151,

15 "Genome screening by comparative genomic hybridization" 13 : 405-409, 1997.

16 "Frequent microsatellite alterations of chromosomelocus 4q13.1 in oral squamous cell carcinomas." 34 : 209-213, 2005.

17 "Fhit expression is absent or reduced in a subsetof primary head and neck cancer." 20 : 1057-1060, 2000.

18 "Evaluation of genetic patterns in different tumor areas of intermediate-grade prostatic adenocarcinomas by high-resolution genomic array analysis." 39 : 249-256, 2004.

19 "Establishment and characterization of human laryngealsquamous cell carcinoma cell lines." 109 : 976-982, 1999.

20 "Dual role of VEGF family members in the pathogenesis of head and neck cancer (HNSCC): possible link between angiogenesis and immune tolerence" 11 : 280-292, 2005.

21 "DNA copynumber gains in head and neck squamous cell carcinomas." 25 : 1424-1433, 2006.

22 "Comparisonof chromosomal imbalances in neuroendocrine and non-small cell lung carcinomas." 114 : 22-30, 1999.

23 "Comparative genomic hybridization as a tool in tumourcytogenetics." 187 : 385-395, 1999.

24 "Common fragile sites, extremely large genes, neural development and cancer." 232 : 48-57, 2006.

25 "Chromosomal and gene amplification in diffuse large Bcell lymphoma" 92 : 234-240, 1997.

26 "Chromosomal alterations in squamous cell carcinomasof the head and neck Window to the biology of disease." 23 : 238-253, 2001.

27 "Cancer Statistics, 1998" 48 : 6-29, 1998.

28 "Biology of SNU cell lines." 37 : 1-19, 2005.

29 "Amplifica-tion of chromosmal region 20q13 in invasive breast cancer: prognostic implications." 1 : 1456-1461, 1995.

30 "Allelic imbalance on chromosome 3p in oral dysplastic lesions: an early event in oral carcinogenesis." 56 : 1228-1231, 1996.