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      Formation of nanostructured poly(lactic-co-glycolic acid)/chitin matrix and its cellular response to normal human keratinocytes and fibroblasts

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      https://www.riss.kr/link?id=A76262586

      • 저자

        Min, Byung-Moo (Department of Oral Biochemistry and Dental Research Institute, IBEC and BK21 HLS, College of Dentistry, Seoul National University) ;  You, Young (Department of Textile Engineering, Chungnam National University) ;  Kim, Jin-Man (Department of Oral Biochemistry and Dental Research Institute, IBEC and BK21 HLS, College of Dentistry, Seoul National University) ;  Lee, Seung-Jin (College of Pharmacy, Ewha Womans University) ;  Park, Won-Ho (Department of Textile Engineering, Chungnam National University)

      • 발행기관
      • 학술지명
      • 권호사항
      • 발행연도

        2004

      • 작성언어

        English

      • KDC

        518

      • 자료형태

        학술저널

      • 수록면

        100-107(8쪽)

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      다국어 초록 (Multilingual Abstract)

      Electrospinning of poly(lactic-co-glycolic acid) (PLGA) and chitin in I,I, 1,3,3,3-hexafluoro-2-propanol (HFIP) and formic acid. respectively. was investigated to fabricate a biodegradable nanostructured composite matrix for tissue engineering. The average fiber diameter (310 nm) of PLGA nanofibers, obtained by electrospinning a 15 wt% PLGA solution in polar HFIP, was much smaller than the diameter (760 nm) of nanofibers electrospun at the same concentration in nonpolar chloroform. On the contrary, chitin was electrospun into nanosized particles at a lower concentration with negligible chain entanglement, because it can not produce continuous fibers by the electrospinning process even at high concentrations. In order to fabricate the biomimetic scaffolds for human keratinocytes and fibroblasts. we prepared the composite matrix (PLGA/chitin = 80/20, w/w) of chitin nanoparticles embedded within a PLGA nanofibcr matrix by a newly-designed simultaneous electrospinning process using two polymer solutions. Chitin nanoparticles were distributed uniformly in the PLGA fibrous structure, and appeared to adhere strongly to PLGA nanofibers by simultaneous electrospinning. To assay the cytocompatibility and cell behavior on the PLGA and PLGA/chitin matrices, cell attachment and spreading of both normal human keratinocytes and fibroblasts seeded on the matrices were studied. Our results indicate that the PLGA/chitin composite matrix may be a better candidate than the PLGA matrix in terms of cell adhesion and spreading for normal human keratinocytes. and that the PLGA and PLGA/chitin matrices are good matrices for normal human fibroblasts.
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      Electrospinning of poly(lactic-co-glycolic acid) (PLGA) and chitin in I,I, 1,3,3,3-hexafluoro-2-propanol (HFIP) and formic acid. respectively. was investigated to fabricate a biodegradable nanostructured composite matrix for tissue engineering. The av...

      Electrospinning of poly(lactic-co-glycolic acid) (PLGA) and chitin in I,I, 1,3,3,3-hexafluoro-2-propanol (HFIP) and formic acid. respectively. was investigated to fabricate a biodegradable nanostructured composite matrix for tissue engineering. The average fiber diameter (310 nm) of PLGA nanofibers, obtained by electrospinning a 15 wt% PLGA solution in polar HFIP, was much smaller than the diameter (760 nm) of nanofibers electrospun at the same concentration in nonpolar chloroform. On the contrary, chitin was electrospun into nanosized particles at a lower concentration with negligible chain entanglement, because it can not produce continuous fibers by the electrospinning process even at high concentrations. In order to fabricate the biomimetic scaffolds for human keratinocytes and fibroblasts. we prepared the composite matrix (PLGA/chitin = 80/20, w/w) of chitin nanoparticles embedded within a PLGA nanofibcr matrix by a newly-designed simultaneous electrospinning process using two polymer solutions. Chitin nanoparticles were distributed uniformly in the PLGA fibrous structure, and appeared to adhere strongly to PLGA nanofibers by simultaneous electrospinning. To assay the cytocompatibility and cell behavior on the PLGA and PLGA/chitin matrices, cell attachment and spreading of both normal human keratinocytes and fibroblasts seeded on the matrices were studied. Our results indicate that the PLGA/chitin composite matrix may be a better candidate than the PLGA matrix in terms of cell adhesion and spreading for normal human keratinocytes. and that the PLGA and PLGA/chitin matrices are good matrices for normal human fibroblasts.

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      목차 (Table of Contents)

      • Abstract
      • 1. Introduction
      • 2. Experimental
      • 2.1. Materials
      • 2.2. Preparation of regenerated chitin
      • Abstract
      • 1. Introduction
      • 2. Experimental
      • 2.1. Materials
      • 2.2. Preparation of regenerated chitin
      • 2.3. Electrospinning
      • 2.4. Measurements
      • 2.5. Cells and cell culture
      • 2.6. Cell adhesion assay and cell spreading analysis
      • 3. Results and discussion
      • 3.1. Electrospinning of PLGA and regenerated chitin
      • 3.2. Composite electrospinning of PLGA/chitin
      • 3.3. Attachment and spreading of normal human keratinocytes and fibroblasts on PLGA and PLGA/chitin matrices, alone or in conjunction with ECM protein coating
      • 4. Conclusions
      • Acknowledgement
      • References
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