Expression of a Functional Type-I Chalcone Isomerase Gene Is Localized to the Infected Cells of Root Nodules of Elaeagnus umbellata

다국어 초록 (Multilingual Abstract)

A putative type-I chalcone isomerase (CHI) cDNA clone EuNOD-CHI was previously isolated from the root nodule of Elaeagnus umbellata [Kim et al. (2003)]. To see if it encodes a functional CHI, we ectopically overexpressed it in the Arabidopsis (Arabidopsis thaliana) transparent testa 5 (tt5) mutant, which is defective in naringenin production and has yellow seeds due to proanthocyanidin deficiency. Ectopic overexpression of EuNOD-CHI resulted in recovery of normal seed coat color. Naringenin produced by CHI from naringenin chalcone was detected in the transgenic lines like in the wild-type, whereas it was absent from the tt5 mutant. We conclude that EuNOD-CHI encodes a functional type-I CHI. In situ hybridization revealed that EuNODCHI expression is localized to the infected cells of the fixation zone in root nodules

참고문헌 (Reference)

1 "Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase" 7 : 786791-, 2000

2 "Polymorphism and expression of isoflavone synthase genes from soybean cultivars" 19 : 6773-, 2005

3 "Integration of signalling pathways in the establishment of the legume-rhizobia symbiosis" 123 : 207218 -, 2005

4 "In situ localization of specific mRNAs in plant tissues in Methods in Plant Molecu-lar Biology and Biotechnology" CRC press 179205-, 1993

5 "Genetics and biochemistry of sed flavonoids" 57 : 405430-, 2006

6 "Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabi-dopsis thaliana" 16 : 735743-, 1998

7 "Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis" 126 : 524535-, 2001

8 "Flavonoid-like compounds from seds of red alder (Alnus rubra) influence host nodula-tion by Frankia (Actionmycetes)" 99 : 588-593, 1997

9 "Flavan-containg cels delimit Frankia-infected compartments in Casuarinas glauca nodules" 121 : 113122-, 1999

10 "Expressed sequence-tag analysis in Casuarina glauca actinorhizal nodule and root" 169 : 681688-, 2006

11 "Differential expression patterns Ho Bang Kim et al. 409 .of an acidic chitinase and a basic chitinase in the rot nodule of Elaeagnus umbellata" 15 : 209215-, 2002

12 "Developmental biology of legume nodula-tion. New Phytol. 122" 1992

13 "Comparison of resveratrol, SOD activity, phenolic compounds and free amino acids in Rehmannia glutinosa un-der temperature and water stress" 56 : 4453-, 2006

14 "Chalcone synthase transcripts are detected in alfalfa root hairs folowing inoculation with wild-type Rhizobium meliloti" 10 : 5058-,

15 "Chalcone reductase-homologous transcripts acumulate during development of stem-borne nodules on the tropical legume Sesbania rostrata" 209 : 45-52, 1999

16 "Chalcone isomerase isozymes with diferent substrate specificities toward 6′-hydroxy and 6′-deoxychalcones in cultured cells of Glycyrhiza echinata, a leguminous plant producing 5′-deoxyflavo-noids" 42 : 11691173-, 2001

17 "Analysis of plant gene expresion" IRL press 135-, 1988

18 "An Arabidopsis short root and dwarfism mutant de-fines a novel locus that mediates both cell division and elon-gation" 49 : 6169-, 2006

19 "Alltechs Chromatography Sourcebook Bulletin #460A" Alltech Associates, Inc., Deerfield 2006

20 "A type-I chalcone isomerase mRNA is highly expressed in the rot nodules of Elaeagnus umbellata" 46 : 263270-,

21 "A set of plant expression vectors for transcrip-tional and translational fusions. Nucleic Acids Res. 15" 5890-, 1987

22 "A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy(iso)flavonoids in Lotus japoni-cus" 131 : 941951-,