MCF-7 유방암세포에서 Resveratrol과 Quercetin의 병행처리에 의한 AMPK 활성 및 시너지 효과 = The Synergistic Effect of Combined Treatment of Resveratrol and Quercetin on AMPK Activity in MCF-7 Breast Cancer Cells

다국어 초록 (Multilingual Abstract)

pathway in many cancer cells. Resveratrol and quercetin have been known to exert anti-inflammatory
or anti-cancer effects through regulating several pathways. AMP-activated protein kinase (AMPK) is one
of the most important protein kinases involved in signal pathways related to metabolic control. AMPK
is regulated by AMP/ATP ratio and activated by ATP consumption. Recently, it has been reported that
AMPK is involved in the regulation of proliferation and/or apoptosis in several cancer cells. Our study
investigated the synergistic effects of resveratrol and quercetin on apoptosis and AMPK activation in
MCF-7 breast cancer cells. Treatment with resveratrol and quercetin or co-treatment with resveratrol and
quercetin for 24 h inhibited cancer cell proliferation, and increased apoptotic cell death. The synergistic
effects of resveratrol and quercetin were observed both in the inhibition of cell growth and the acceleration
of apoptotic cell death. Also, the combined treatment of resveratrol and quercetin more strongly activated
AMPK, and this effect was abolished by AMPK inhibitor Compound C in MCF-7 breast cancer cells.
Treatment of AICAR, synthetic AMPK activator also increased phospho-AMPK levels. Our results suggest
that combined treatment of resveratrol and quercetin is a more effective way in controlling cancer
proliferation and apoptosis. (Cancer Prev Res 14, 218-224, 2009)

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