Arbutin , Glycolic Acid , Kojic Acid 및 Pentadecenoic Acid가 in vitro 및 in vivo에서 UVB 조사에 의한 색소형성에 미치는 영향 = The Effect of Arbutin , Glycolic Acid , Kojic Acid and Pentadecenoic Acid on the in vitro and in vivo Pigmentary System After Ultraviolet - B ( UVB ) IrradiationArbutin , Glycolic Acid , Kojic Acid 및 Pentadecenoic Acid가 in vitro 및 in vivo에서 UVB 조사에 의한 색소형성에 미치는 영향

다국어 초록 (Multilingual Abstract)

Background:Melanin pigmentation palys a major role in normal skin color. The rates of melanin synthesis by melanocytes appear to be regulated by ultraviolet-B UVB) rediation and chemicals, though the precise mechanisms modulating human epidermal pigmentation are unknown. Several chemicals including arbutin, kojic acid(KA), pentadecen?ic acid(PDA) and glycolic acid(GA) have been suggested as a melanogenesis inhibitory compounds because of their chemical or biological similarities with hydroquinone or their tyrosinase inhibitory effcet. Objective:The purpose of this study was to evaluate the inhibitory effect of arbutin, GA, KA and PDA on UV-induced melanogenesis in the in vitro and in vivo pigmentary system. Methods:Cultured normal melanocytes and B-16 melanoma cells, and C57BL mice and human volunteers were used for in vitro and in vivo studies respectively. They were administered to UVB irradiated or nonirradiated cultured normal human melanocytes, and B-16 melanoma cells. For the in vivo study, these chemicals were topically applied to C57BL mice and human ?olunteer skin after UVB irradiation. Numeric and morphologic changes and melanin content were measured in cultured normal human melanocytes and B-16 melanoma cells. In the C57BL mice, numeric and morphologic changes of split-DOPA stained melanocytes were assessed. In the human volunt?rs, gross pigementary changes wre evaluated. Results 1. The number and melanin content of cultured melanocytes initially decreased after UVB-irradiation, but the melanin content increased 5 days after irradiation. 2. Cell numbers of irradiated or nonirradiated cultured human melanocytes decreased in arbutin(10^-3M), KA(10^-3M, 10^-5M), PDA(10^-3M) groups. Those of the cultured B-16 mealnoma cells decreased only in the arbutin(10^-3M) group after UVB irradiation. 3. Melanin contents of cultured human melanocytes decreased in crbutin(10^-3M, 10^-5M), KA(10^-3M, 10^-5M) and PDA(10^-3M) groups. Those of cultured B-16 melanon a cells decreased in arbutin(10^-3M, 10^-5M) groups after UVB-irradiation or nonirradiation. 4. The number of split-DOPA(+) melanocytes decreased in the groups ?reated with KA 1% for 3,5 and 7 weeks, KA 0.1%, arbutin 3%, arbutin 5% for 5and 7 qwwks and PDA 5.0% for 7 weeks in the C57BL mice. 5. The number of split-DOPA(+) melanocytes decreased in the groups ?reated with KA 1.0%, PDA 5.0%, arbutin 3% and arbutin 5% for 5 and 7 weeks and KA 0.1% for 7 weeks in UVB irradiated C57BL mice. 6. Visible inhibition of UVB-induced hyperpigmentation was observed in arbutin applied sites in 4 of the 6 volunteers 3 weeks after the application. GA did not show an inhibitory effect on UVB-induced hyperpigmentation in all subjects. Conclusion:Arbutin, KA, PDA had a suppressive effect on ? of nonirradiated melanocytes and melanoma cells as well as UVB-induced hyperpigmentation. It is suggested that these drugs might be candidates as compounds that may control hyperpigmentary disorders.(Kor J Dermatol 1994;32(6):977~989)