柴胡의 난소암세포 SKOV-3에 대한 항암효과

다국어 초록 (Multilingual Abstract)

Anti-cancer effects of
Bupleurum falcatum L Ethanol Extract
in Ovarian cancer cell SKOV-3 cells

Jin Young Hur
Dept. of Clinical Korean Medicine
Graduate School
Kyung Hee University
Seoul, Korea
(Supervised by Prof. Jun Bock Jang, K.M.D., Ph.D.)

Objectives: Bupleurum falcatum L Ethanol Extract (BEE) is a species of flowering plant and has been widely used in eastern asia to treat various diseases. The anti-cancer-related molecular mechanisms by BEE are not yet well understood. We investigated and identified that BEE is a powerful anti-cancer therapeutic strategy in SKOV-3 ovarian cancer cell line.

Methods: The effects of the BEE on cell viability and cell cytotoxicity were performed using WST-1 and LDH assay. Western blotting analyses were performed to identify caspase-3 cleavage level in SKOV-3 cell lines. Z-VAD-FMK in combiantion with Bupleurum falcatum L were investigated to evaluate caspase-3-dependent anti-cancer effect in SKOV-3 cell lines.

Results: BEE exerts the reduction of cell viatbility and the increase of cell cytotoxicity via the caspase-3 cleavage in SKOV-3 cell lines dose- and time-dependently.

Conclusion: BEE mediated the apoptosis in SKOV-3 ovarian cancer cell lines. BEE may expect as a potential candidate for on the treatment for ovarian cancer patients.

목차 (Table of Contents)

• Ⅰ. 서론 1
• Ⅱ. 실험 3
• 1. 재료 3
• 1) 약물 3
• 2) 시약 3
• 3) 세포주와 세포배양 3
• 2. 방법 4
• 1) 세포생존률 측정 4
• 2) 세포독성 측정 4
• 3) Western blotting 5
• 3. 통계처리 5
• Ⅲ. 결과 6
• 1. BEE 농도별 세포생존률 효과 6
• 2. BEE 농도별 세포독성 효과 7
• 3. BEE의 시간의존 세포생존률 효과 8
• 4. BEE의 시간의존 세포독성 효과 9
• 5. BEE의 농도의존적 Caspase-3 cleavage 발현 효과 10
• 6. BEE의 시간의존적 Caspase-3 cleavage 발현 효과 11
• 7. BEE와 Z-VAD-FMK의 병용처리 후 세포생존률 효과 12
• 8. BEE와 Z-VAD-FMK의 병용처리 후 세포독성 효과 13
• 9. BEE와 Z-VAD-FMK의 병용처리 후 Caspase-3 cleavage 발현 효과 14
• Ⅳ. 고찰 15
• Ⅴ. 결론 20
• Ⅵ. 참고문헌 22
• ABSTRACT 26