본 연구의 목적은 PGC-1α의 짧은 형태인 NT-PGC-1α가 골격근 내 미토콘드리아 생합성에 어떠한 영향을 미치는가를 알아보는 것이다. 지구성 운동에 따른 골격근 내 PGC-1α, NT-PGC-1α 그리고 미토�...
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https://www.riss.kr/link?id=A99578775
2012
Korean
PGC-1α ; NT-PGC-1α ; 골격근 ; C2C12 ; 지구성 운동 ; 미토콘드리아 생합성 ; PGC-1α ; NT-PGC-1α ; skeletal muscle ; C2C12 ; endurance exercise ; mitochondrial biogenesis
692
KCI등재,SCOPUS
학술저널
435-443(9쪽)
4
0
상세조회0
다운로드국문 초록 (Abstract)
본 연구의 목적은 PGC-1α의 짧은 형태인 NT-PGC-1α가 골격근 내 미토콘드리아 생합성에 어떠한 영향을 미치는가를 알아보는 것이다. 지구성 운동에 따른 골격근 내 PGC-1α, NT-PGC-1α 그리고 미토�...
본 연구의 목적은 PGC-1α의 짧은 형태인 NT-PGC-1α가 골격근 내 미토콘드리아 생합성에 어떠한 영향을 미치는가를 알아보는 것이다. 지구성 운동에 따른 골격근 내 PGC-1α, NT-PGC-1α 그리고 미토콘드리아 생합성 정도를 알아보기 위해 18마리의 흰쥐를 각각 6마리씩 3그룹(sedentary; Sed, 1 day exercise; 1d Ex, 3 day exercise; 3d Ex)으로 분류한 다음 수영을 실시(6 h/day)하였다. 골격근은 마지막 운동훈련 18시간후 적출하였다. 그 결과 지구성 운동에 따른 골격근 내 PGC-1α의 발현은 1일 운동 후에는 Sed 그룹에 비해 유의하게 증가 하였으나, 3일 운동 후에는 1일 운동에 의해 증가된 정도가 Sed 그룹 수준으로 감소하였다. NT-PGC-1α는 1일 운동 후에는Sed 그룹과 비교해 차이가 없었으나, 3일 운동 후 유의하게 증가하였다. 그리고 지구성 운동에 따른 골격근 내 COXⅠ과NADH-UO는 1일과 3일 트레이닝 후 모두 유의하게 증가하였다. 또한 NT-PGC-1α가 미토콘드리아 생합성에 어떠한 영향을 미치는지 알아보기 위해 C2C12 골격근 세포에 NT-PGC-1α adenovirus를 처치하여 NT-PGC-1α를 과발현 시킨 후 미토콘드리아 효소의 발현정도를 분석한 결과 미토콘드리아 효소인 cytochrome c, COXⅠ, ATP synthase, citrate synthase 모두 유의하게 증가하였다. 이러한 결과를 종합해 볼 때, PGC-1α는 미토콘드리아 생합성 초기에 관여하고, NT-PGC-1α는 PGC-1α에 의해증가된 미토콘드리아 생합성을 계속 유지하거나 약간 더 증가시키는데 중요한 역할을 할 것이다.
다국어 초록 (Multilingual Abstract)
N-truncated peroxisome proliferator-activated receptor γ coactivator-1α splice variant (NT-PGC-1 α) has similar biological effects to peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α) in brown adipose t issue. But, t he e ffect...
N-truncated peroxisome proliferator-activated receptor γ coactivator-1α splice variant (NT-PGC-1 α) has similar biological effects to peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α) in brown adipose t issue. But, t he e ffect of NT-PGC-1α on mitochondrial biogenesis in skeletal muscles has not been clearly identified. The purpose of this study is to verify that NT-PGC1 induce the mitocondrial biogenesis in skeletal muscle. Eighteen rats were randomly assigned into 3 groups (Sedentary, Sed; 1 day exercise, 1d Ex; 3 day exercise, 3d Ex). Exercise groups were trained by using a 6 h/day swimming program. Muscle samples for PGC-1α, NT-PGC-1α and mitochondrial enzymes were harvested 18 hours after the last bout of exercise. The expression of PGC-1α was increased in the 1d Ex compared with Sed group, but 3d Ex group was not changed compared with Sed group. NT-PGC-1α was increased in the 3d Ex group from the Sed group. The expressions of cytochrome oxidase subunit I (COXⅠ)and NADH-ubiquinone oxidoreductase (NADH-UO) were increased in the 1d Ex and 3d Ex group from the Sed group. Three day overexpressed NT-PGC-1α C2C12 myotubes were harvested for mitochondrial enzymes. The expressions of mitochondrial enzymes, cytochrome c, COXⅠ, ATP synthase and citrate synthase were increased in the NT-PGC-1α overexpressed C2C12 from the Sed group. These findings suggest that PGC-1α protein is responsible for initial increase in mitochondrial biogenesis. While NT-PGC-1α is important for chronic exercise induced increase in mitochondrial proteins.
참고문헌 (Reference)
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정상체중아동과 비만아동의 인슐린저항성지표 및 혈청 비스파틴 수준 비교
고강도 복합 트레이닝이 사이클 선수의 체력 및 4 km 기록에 미치는 영향
아동의 PPARGC1A 유전자형(Gly482Ser)과 신체활동, 비만지표, 대사성위험인자와의 연관성
노화가 평형성 유지 시 운동조절 기능 및 근활성도에 미치는 영향
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2026 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2020-01-01 | 평가 | 등재학술지 유지 (재인증) | |
2017-01-01 | 평가 | 등재학술지 유지 (계속평가) | |
2013-01-01 | 평가 | 등재 1차 FAIL (등재유지) | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2008-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2006-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2005-03-21 | 학회명변경 | 한글명 : 한국운동과학회 -> 한국운동생리학회영문명 : Korea Exercise Science Academy -> Korean Society of Exercise Physiology | |
2005-03-21 | 학회명변경 | 한글명 : 한국운동과학회 -> 한국운동생리학회영문명 : Korea Exercise Science Academy -> Korean Society of Exercise Physiology | |
2003-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2002-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2001-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.76 | 0.76 | 0.67 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.62 | 0.71 | 0.674 | 0.03 |