Haloperoxidase를 생산하는 미생물을 분리하기 위하여 국내 연근해와 남북극 등의 해양시료에서 분리된 방선균 균주를 대상으로 탐색을 수행하여 남해 백도 해조류 추출물로부터 분리된 한 종...
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https://www.riss.kr/link?id=A101546572
조기웅 (안양대학교) ; Cho, Ki-Woong
2008
Korean
KCI등재,SCOPUS
학술저널
116-121(6쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
Haloperoxidase를 생산하는 미생물을 분리하기 위하여 국내 연근해와 남북극 등의 해양시료에서 분리된 방선균 균주를 대상으로 탐색을 수행하여 남해 백도 해조류 추출물로부터 분리된 한 종...
Haloperoxidase를 생산하는 미생물을 분리하기 위하여 국내 연근해와 남북극 등의 해양시료에서 분리된 방선균 균주를 대상으로 탐색을 수행하여 남해 백도 해조류 추출물로부터 분리된 한 종류의 방선균(#1460)에서 높은 haloperoxidase 활성이 확인되었다. 본 균주의 생리.생화학적 특성은 Streptomyces 속과 유사하며 생산되는 haloperoxidase는 세포 조 추출물로부터 ammonium sulfate precipitation, High-Q column chromatography, gel permeation chromatography, Hydroxyapetite chromatography 그리고 hydrophobic interaction chromatography를 통하여 42%의 수율과 purification fold 70으로 정제하였다. 본 효소의 최적 반응 pH는 7이고 pH 8에서 더 높은 안정성을 보여 $60^{\circ}C$에서 1시간 반응에 효소활성의 50%가 생존한다. 또 cyanide와 azide 이온에 의해 강한 저해현상을 보인다.
다국어 초록 (Multilingual Abstract)
In my search of microbial source of novel enzymes, a marine actinomycetes, A1460, producing haloperoxidase was isolated from macroalgae from south sea, Korea and studied for physiological and biochemical properties. The haloperoxidation reaction was f...
In my search of microbial source of novel enzymes, a marine actinomycetes, A1460, producing haloperoxidase was isolated from macroalgae from south sea, Korea and studied for physiological and biochemical properties. The haloperoxidation reaction was followed by the bromination of phenol red in the presence of hydrogen peroxide and potassium bromide. The haloperoxidase was partially purified from the cell extract with $35\sim75%$ ammonium sulfate precipitation, High-Q anion exchange chromatography, gel filtration chromatography, hydroxyapetite chromatography and hydrophobic interaction chromatography to a yield of 42% and purification fold of 70. This enzyme showed relatively high heat stability without losing 50% of activity after 1 hr incubation at $60^{\circ}C$. The highest activity was found at $45^{\circ}C$, and the optimal pH was about pH 7, but higher stability was observed at pH 8. Azide and cyanide ion showed strong inhibition at less than 1 $\mu M$ level suggesting that the enzyme was Fe ion dependent haloperoxidase.
참고문헌 (Reference)
1 Kirner, S., "The non-haem chloroperoxidase from Pseudomonas fluorescens and its relationship to pyrrolnitrin biosynthesis" 142 : 129-135, 1996
2 Hunter-Cevera, J.C., "Screening for a new enzyme in nature: Haloperoxidase production by death valey dematiaceous Hyphomycetes" 12 : 121-127, 1986
3 Rush, C., "Purification, crystallisation and preliminary X-ray analysis of the vanadium-dependent haloperoxidase from Corallina officinalis" 359 : 244-246, 1995
4 Wiesner, W., "Purification and characterization of a novel bacterial non-heme chloroperoxidase from Pseudomonas pyrrocinia" 263 : 13725-13732, 1988
5 Butler, A, "Marine haloperoxidases" 93 : 1937-1944, 1993
6 Littlechild, J, "Haloperoxidases and their role in biotransformation reactions" 3 : 28-34, 1999
7 Geigert, J., "Epoxidation of alkenes by chloroperoxidase catalysis" 136 : 778-782, 1986
8 Pelletier, I., "Cloning of a second non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762: sequence analysis, expression in Streptomyces lividans and enzyme purification" 140 : 509-516, 1994
9 Dairi, T., "Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline. Biosci" 59 : 1099-1106, 1995
10 Wolfframm, C., "Chloroperoxidase-encoding gene from Pseudomonas pyrrocinia: sequence, expression in heterologous hosts, and purification of the enzyme" 130 : 131-135, 1993
1 Kirner, S., "The non-haem chloroperoxidase from Pseudomonas fluorescens and its relationship to pyrrolnitrin biosynthesis" 142 : 129-135, 1996
2 Hunter-Cevera, J.C., "Screening for a new enzyme in nature: Haloperoxidase production by death valey dematiaceous Hyphomycetes" 12 : 121-127, 1986
3 Rush, C., "Purification, crystallisation and preliminary X-ray analysis of the vanadium-dependent haloperoxidase from Corallina officinalis" 359 : 244-246, 1995
4 Wiesner, W., "Purification and characterization of a novel bacterial non-heme chloroperoxidase from Pseudomonas pyrrocinia" 263 : 13725-13732, 1988
5 Butler, A, "Marine haloperoxidases" 93 : 1937-1944, 1993
6 Littlechild, J, "Haloperoxidases and their role in biotransformation reactions" 3 : 28-34, 1999
7 Geigert, J., "Epoxidation of alkenes by chloroperoxidase catalysis" 136 : 778-782, 1986
8 Pelletier, I., "Cloning of a second non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762: sequence analysis, expression in Streptomyces lividans and enzyme purification" 140 : 509-516, 1994
9 Dairi, T., "Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline. Biosci" 59 : 1099-1106, 1995
10 Wolfframm, C., "Chloroperoxidase-encoding gene from Pseudomonas pyrrocinia: sequence, expression in heterologous hosts, and purification of the enzyme" 130 : 131-135, 1993
11 Bantleon, R., "Chloroperoxidase from Streptomyces lividans: isolation and characterization of the enzyme and the corresponding gene" 176 : 2339-2347, 1994
12 Calbiochem, "Calbiochem manual for lactoperoxidase" CA 2007
13 Van Pee, K.H., "Biosynthesis of halogenated metabolites by bacteria. Annu" 50 : 375-399, 1996
14 Pelletier, I, "A bacterial esterase is .homologous with non-haem haloperoxidases and displays brominating ctivity" 141 : 459-468, 1995
15 Kataoka, M., "4-Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46" 267 : 3-10, 2000
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서울지역 급성위장관염 환자에서 검출된 노로바이러스의 유전자형 분포
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2013-12-02 | 학술지명변경 | 외국어명 : The Korean Journal of Microbiology -> Korean Journal of Microbiology | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2008-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2006-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2001-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
1998-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.21 | 0.21 | 0.21 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.26 | 0.24 | 0.48 | 0.02 |