꾸지 뽕나무 휘발성 향기성분의 항산화활성

국문 초록 (Abstract)

꾸지 뽕나무(C. tricuspidata)의 휘발성 향기추출물의 항산화활성을 2 가지의 시험방법을 이용하여 측정하였다. 그리고 꾸지 뽕나무의 향기추출물에서 동정된 10종의 휘발성 향기성분에 대한 항산화활성도 측정하였다. 꾸지 뽕나무의 줄기 및 뿌리 향기추출물들은 2가지 실험법에서 모두 뚜렷하게 처리농도에 비례하여 항산화활성을 나타내었다. 즉 aldehyde/carboxylic acid assay에서 500 ㎍/mL의 농도로 처리하였을 때, 줄기와 뿌리의 향기추출물은 각각 77.02±8.12%와 74.19±6.82%의 항산화활성을 나타내었으며, lipid/malonaldehyde assay에서 160 ㎍/mL의 농도로 처리하였을 때, 줄기 및 뿌리의 향기추출물은 각각 61.43±2.11 및 76.17±4.25%의 항산화활성을 나타내었다. 꾸지 뽕나무의 향기추출물에서 동정된 향기성분들은 7종의 terpenes and terpenoides, 14종의 alkyl compounds, 11종의 nitrogen containing heterocyclic compounds, 3종의 oxygen containing heterocyclic compounds, 12종의 aromatic compounds, 9종의 lactones와 7종의 miscellaneous compounds였다. 꾸지뽕나무의 향기추출물에서 동정된 향기성분들 중에서 eugenol, isoeugenol 및 2,4-bis(1,1-dimethylethyl)phenol은 lipid/malonaldehyde assay에서 160 ㎍/mL의 농도로 처리하였을 때, 각각 91.74±1.33, 94.00±0.59 및 91.22±4.74%의 항산화활성을 나타내었다. 이는 동일한 방법에서 BHT와 α-tocopherol의 항산화활성이 각각 91.90±0.42%와 89.47±3.04%인 것과 비슷한 수준이었다. 한편 동일한 처리농도에서 vanillin과 2-acetylpyrrole은 각각 63.36±5.83과 58.62±6.83%로서 중간 정도의 항산화활성을 나타내었다. 따라서 꾸지 뽕나무는 산화적 손상으로 인한 질병의 예방과 사람들의 건강에 도움을 줄 수 있을 것으로 생각되었다.

다국어 초록 (Multilingual Abstract)

The antioxidant activities of volatile aroma extracts from Cudrania tricuspidata (Carr.) Bureau were examined using two antioxidant assays. Ten volatile aroma compounds identified in this plant were also tested for antioxidant activity. The volatile aroma extracts of stem and root from C. tricuspidata exhibited antioxidant activities with a clear dose response relationship in both aldehyde/carboxylic acid and lipid/malonaldehyde assays. Antioxidant activities of volatile aroma extracts from C. tricuspidata at 500 ㎍/mL were 77.02±8.12% (stem) and 74.19±6.82% (root) in the aldehyde/carboxylic acid assay. Antioxidant activities of volatile aroma extracts from C. tricuspidata at 160 ㎍/mL were 76.17±4.25% (stem) and 61.43±2.11% (root) in the lipid/malonaldehyde assay. Positively identified volatile aroma components in extracts of stem and root from C. tricuspidata were seven terpenes and terpenoides, 14 alkyl compounds, 11 nitrogen containing heterocyclic compounds, three oxygen containing heterocyclic compounds, 12 aromatic compounds, nine lactones, and seven miscellaneous compounds (possible contaminants). Among the positively identified compounds, eugenol, isoeugenol, and 2,4-bis (1,1-dimethylethyl)phenol exhibited antioxidant activities comparable to those of BHT and α-tocopherol. Vanillin and 2-acetylpyrrole showed moderate activities in the lipid/malonaldehyde assay. These results suggest that consumption of antioxidant-rich beverages prepared from C. tricuspidata could have beneficial effects on human health by preventing diseases caused by oxidative damage.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

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