차가버섯의 온도단계별 물추출물의 항산화성 비교

국문 초록 (Abstract)

각종 산소 라디칼들이 생체내의 호흡효소계나 산화환원계에서 생성되어 노화와 각종 질병과 유관한 사실이 밝혀져감에 따라 이들의 소거를 위한 항산화물질에 대한 관심이 높아지고 있는 시점에서, 본 논문에서는 차가버섯의 대표 유효성분인 항산화 성분을 최대로 추출해내기 위한 효과적인 추출법을 시도하고자 실행하였다. 차가버섯 건조분말을 80℃에서 8시간 추출한 것을 1차 추출물, 1차 추출 후 그 잔사물에 물을 첨가하여 100℃에서 8시간 추출한 것을 2차 추출물, 2차 추출 후 남은 잔사물에 물을 첨가하여 120℃에서 8시간 추출한 것을 3차 추출물로 하여서, DPPH 자유 라디칼에 대한 전자공여능, ABTS·+ 라디칼 양이온 소거능, 리놀레산 자동산화 저해 효과를 봄으로서 항산화능을 측정하였다. 결과에서 항산화력은 1차 추출물이 가장 낮았고 3차 추출물이 가장 높았고, 3차 추출물은 페놀 화합물과 플라보노이드 함량이 1차나 2차 추출물보다 유의적으로 많았고, 페놀화합물 함량은 라디칼 소거능 및 리놀레산 자동산화 저해효과와 상관관계가 높은 것(r=0.960~0.980, regression analysis)으로 나타났다. 또한 각 온도에서의 추출물을 혼합하여 지방산화 촉진인자인 3종의 활성산소종(KO₂, H₂O₂, ㆍOH)과 금속 이온(Fe^(2+), Cu^(2+)) 존재 하에서 항산화 효과를 TBARS값으로 측정한 결과, 차가추출물이 모두의 경우에서 TBARS값을 낮추었으며 특히 ㆍOH와 Fe^(2+) 이온에 대하여 탁월한 항산화능을 보였다. 이상의 결과로 미루어 보아 자유 라디칼로 인해 야기되는 지방을 비롯한 그 산화물에 대하여 3단계 온도 추출법에 의한 차가버섯 추출물은 매우 유효한 항산화제로서 작용할 수 있을 것으로 사료되어진다.

다국어 초록 (Multilingual Abstract)

The efficacy of extraction from Inonotus obliquus was examined from the points of antioxidative characteristics and some antioxidative compounds. To enhance the efficient extraction for the effective components from Inonotus obliquus, temperature-stepwise water extraction method was applied. Temperature-stepwise water extracts were prepared for 8 hrs as follows: the first extract at 80℃, the second extract from the residue of the first extract at 100℃, and the third extract from the residue of the second extract at 120℃. Antioxidative activities were determined by electron-donating ability of DPPHㆍ free radical, scavenging ability of ABTSㆍ^+ radical cation, and by inhibiting ability of linoleic acid autoxidation. In results, the first extract showed the least antioxidant capacity, and the third extract showed the highest antioxidant capacity. The third extract also had the greatest amounts of phenolic compounds and flavonoids. Amounts of phenolic compound from each extract were almost proportional to the radical scavenging activities and linoleic acid autoxidation inhibiting ability (r=0.960~0.980, regression analysis). Furthermore, the effect of the pooled extract of all three extractions of Inonotus obliquus on the lipid peroxidation reacted with active oxygen species (KO₂, H₂O₂, ㆍOH) and metals (Fe^(2+), Cu^(2+)) was evaluated by measuring the formation of thiobarbituric acid reactive substances (TBARS). The pooled Inonotus obliquus extracts lowered the amounts of TBARS formed by all of the active oxygen species and metals. Especially, these lowering effects were pronounced in the reaction with ㆍOH and Fe^(2+). These results suggest that the pooled temperature-stepwise extract from Inonotus obliquus could be potential functional materials to reduce the oxidation of lipids and other compounds induced by free radicals.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 감사의 글
  
• 문헌

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