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あぁかがさざただなはばぱまやゃらわゎんいぃきぎしじちぢにひびぴみりうぅくぐすずつづっぬふぶぷむゆゅるえぇけげせぜてでねへべぺめれおぉこごそぞとどのほぼぽもよょろを

アァカサザタダナハバパマヤャラワヮンイィキギシジチヂニヒビピミリウゥクグスズツヅッヌフブプムユュルエェケゲセゼテデヘベペメレオォコゴソゾトドノホボポモヨョロヲ ―

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½ ⅓ ⅔ ¼ ¾ ⅛ ⅜ ⅝ ⅞ ¹ ² ³ ⁴ ⁿ ₁ ₂ ₃ ₄

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RISS 인기검색어

Binary vector system을 이용한 Escherichia coli로의 수은내성 유전자의 전이 = Transfer of Hg^++ -resistance gene into Escherichia coli with binary vector system

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https://www.riss.kr/link?id=A19621363

저자

李浩子 (慶熙大學校文理科大學生物學科) ; 金耕淑 (慶熙大學校文理科大學生物學科)
발행기관
慶熙大學校
학술지명
論文集
권호사항

Vol.18 No.- [1989]
발행연도
1989
작성언어
Korean
KDC
104.000
자료형태
학술저널
수록면

255-272(18쪽)
제공처
RISS
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다국어 초록 (Multilingual Abstract)

A recombinant plasmid was constructed by transferring Hind Ⅲ DNA fragment of pHP 45 : : omega - Hg , including Hg^(++)-resistant gene , into Hind Ⅲ restriction site of pGA 642 and was named pSA 2. pSA 2 was transfered into E.coli S17-1 and the transformant was convinced on selection media.
By the dual mating , the transfer frequency of pGA 642 was increased about 10^3 times than the frequency obtained by the triple mating. The transfer frequency of pGA 642 varied greatly dependent on pH values, and the best transfer frequency was about 2.7 × 10 exp (-3) at pH 5.5. The optical mating time was 36 hrs, and the transfer frequency was 2.9 × 10 exp (-2).

번역하기

A recombinant plasmid was constructed by transferring Hind Ⅲ DNA fragment of pHP 45 : : omega - Hg , including Hg^(++)-resistant gene , into Hind Ⅲ restriction site of pGA 642 and was named pSA 2. pSA 2 was transfered into E.coli S17-1 and the tra...

A recombinant plasmid was constructed by transferring Hind Ⅲ DNA fragment of pHP 45 : : omega - Hg , including Hg^(++)-resistant gene , into Hind Ⅲ restriction site of pGA 642 and was named pSA 2. pSA 2 was transfered into E.coli S17-1 and the transformant was convinced on selection media.
By the dual mating , the transfer frequency of pGA 642 was increased about 10^3 times than the frequency obtained by the triple mating. The transfer frequency of pGA 642 varied greatly dependent on pH values, and the best transfer frequency was about 2.7 × 10 exp (-3) at pH 5.5. The optical mating time was 36 hrs, and the transfer frequency was 2.9 × 10 exp (-2).