Genomic appraisal of Klebsiella PGPB isolated from soil to enhance the growth of barley

다국어 초록 (Multilingual Abstract)

Background PGPR has substituted chemical fertilizers to enhance the nutrient profle of the soil. Although gene encoding for PGP activity is present in PGPB their activity changes in response to conditions.
Objective To study comparative genomics for three Klebsiella strains and their PGPR activity in response to in vitro and soil condition.
Methods We evaluated the activity of three Klebsiella spp. in two diferent conditions, specifc nitrogen-defcient MS media and greenhouse experiment. Applying comparative genomics, genes encoding for PGP traits were identifed from the wholegenome sequencing of the three strains. With the help of the RAST tool kit and functional annotation, a total number of genes encoding for cell wall capsule, nitrogen metabolism, sulfur genes and many other functional groups were identifed. With the help of blast circular genome, similarity between GC content, pseudogene and tRNA was represented. The percentage of gene similarity of SSN1 was generated against BLAST with M5a1 and SGM81. Other methods like synteny alignment and orthologous gene clusters were applied to understand the homologous present in three strains.
Results SSN1 was actively producing the maximum amount of ammonia 10.97±0.29 µmol/mL compared to the other two strains. K. oxytoca M5a1 was considered negative for all PGP traits except ammonia production. The activity of SSN1 was showing a consistent pattern both the conditions whereas M5a1 was only active in vitro condition. Gene encoding for allantoin metabolism allD, allC, allB, allA, allE, allR, allH were identifed in SSN1 and M5a1 but was absent in SGM81. The highest COG was shared between SGM81 and SSN1 predicting a maximum number of similar genes. The nif gene cluster was 98% identical to the M5a1 strain.
Conclusions Comparatively, SSN1 expressed the additional gene for various PGP traits which suggest higher efciency of strain in nitrogen defciency stress.

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