Methylation Pattern of H19 Gene at Various Preimplantation Development Stages of In Vitro Fertilized and Cloned Porcine Embryos

목차 (Table of Contents)

• ABSTRACT
• INTRIDUSTION
• MATERIALS AND METHODS
• oocyte Retrieval and In Vitro Maturation
• In Vitro Fertilization(IVF) of Oocytes
• Preparation of Donor Cells for Nuclear Transfer
• Somatic Cell Nuclear Transfer(SCNT) of Oocytes
• Comparative Sequencing
• Genomic DNA Isolation
• Bisulfite(BS) Treatment and Polymerase Chain Reaction(PCR)
• Cloning and sequencing
• Experimental Designs
• Statistical Analysis
• RESULTS
• Sequence Homology
• Bisulfite Sequencing Analysis of Putative DMRs
• Bisulfite Sequencing Analysis of Pocine IVF and SCNT Embryos
• DISCUSSION
• REFERENCES

참고문헌 (Reference)

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