S-2-Aminoethyl L-Cysteine(ABC), an analog of L-lysine, significantly inhibited the growth of wild type strains of Lactobacillus acidophilus IF0 3205, Lactobacillus acidophilus IF0 13952, Lactobacillus bulgaricus ATCC 11842, and Lactobacillus bulgaricu...
S-2-Aminoethyl L-Cysteine(ABC), an analog of L-lysine, significantly inhibited the growth of wild type strains of Lactobacillus acidophilus IF0 3205, Lactobacillus acidophilus IF0 13952, Lactobacillus bulgaricus ATCC 11842, and Lactobacillus bulgaricus IF0 353g. The growth inhibition of these strains depended on AEC concentration. In presence of l0ug/ml of AEC concentration the growth of these strains was inhibited completely. AEC resistant mutants were induced by N-methyl-N-nitro-N-nitrosoguanidine(NTG) and Ethylmethane sulfonate(EMS) treatment. Almost all resistant mutants produced lysine. Lysine productivity of mutants had no insignificant relation t o method of mutagen treatment. Also lysine productivity of mutants had nothing to do with concentration of AEC. Lysine productivity of lysine analog resistant mutants obtained from first mutation was increased by further mutation. Among the AEC resistant mutants, LaNA-412 was selected as lysine producing mutant. LaNA-4 12 produced 103μg/ml L-lysine.