Oxygen consumption rate of mammalian cells in culture

다국어 초록 (Multilingual Abstract)

Even though alanine was grouped as more produced amino acids, alanine production rates was not significantly changed. From this one can assume that alanine aminotransferase (Ala-AT) activity is not present in Jurkat cells. Gayton (1989) reported that both alanine aminotransferase and aspartate aminotransferase participate in the breakdown of glutamine. Therefore, it can be assumed that the reaction of pyruvate is tightly locked by the conversion to lactate. This can be consumed by the fact that in rotenone treated cells a large portion of their energy depends on this conversion to lactate. Therefore major flux from pyruvate should go not to alanine by alanine aminotransferase but to lactate by lactate dehydrogenase. Cells will attempt to maximize the fraction of glucose that is converted to lactate.
It is still important to note that the NADH/NAD+ ratio of cytoplasm is quite different from the ratio of mitochondrial matrix and cristae (Williamson et al., 1967). Therefore, all the assumptions must be verified by experimental measurements of the ratios of NADH/NADH/NAD+ in cytoplasm and mitochondria at every specific condition(Bergel et al.,1989; Bessho et al., 1989).

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Results and Discussion
• References