Antiallergic Effect of Hizikia fusiformis in an Ovalbumin-Induced Allergic Rhinitis Mouse Model|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Objectives. The extract of Hizikia fusiformis is known to exhibit anticancer, antiatopic and antioxidant activities. We aimed to investigate the extract of H. fusiformis on allergic rhinitis inflammation in a mouse model.
Methods. The 4-week-old BALB/c mice were randomly assigned into four groups: group A, control group (n=9); group B, allergic rhinitis group (n=10); group C (n=10) received 300 mg/kg of H. fusiformis during nasal challenging period; group D (n=10) received 600 mg/kg of H. fusiformis during general sensitization period and 300 mg/kg of H. fusiformis during nasal challenging period. Allergic inflammation was made with ovalbumin (OVA) and alum then challenged intranasally with OVA. H. fusiformis was intraperitoneally administered 3 hours before the OVA administration. Allergic symptom score and the levels of immunoglobulin G1 (IgG1), IgG2a, OVA-specific IgE antibodies, levels of cytokines in the nasal mucosa and in spleen cell culture supernatant, such as tumor necrosis factor alpha (TNF-α), interleukin 4 (IL-4), IL-5, IL-13, and IL-10 were assessed. The percentage of regulatory T cell was analyzed by flow cytometry. Eosinophilic infiltration and goblet cell hyperplasia were also evaluated.
Results. H. fusiformis administered groups C and D showed significant inhibitory effects on nasal symptoms, IL-13 mRNA expression and eosinophil infiltration/goblet cell hyperplasia in the nasal tissue; OVA-specific IgE production in serum (P<0.05). In group D, H. fusiformis treatment downregulated IL-4, IL-5, IL-13, TNF-α, and IL-10 cytokine expression in splenocyte culture as well as significantly decreased IgG2a, IgG1 levels in serum compared with group B (P<0.05). However, the expressions of IL-5, interferon-γ and forkhead box P3 mRNA did not change in groups C and D.
Conclusion. H. fusiformis could induce antiallergic inflammation by suppressing the T-helper type 2 cytokine production (IL-13) locally and systemically, OVA-specific IgE formation, goblet cell hyperplasia, and eosinophilic infiltration in a mouse model of allergic rhinitis. Thus, H. fusiformis could be considered as a potential therapeutic agent in treating allergic rhinitis.

번역하기

참고문헌 (Reference)

1 Kanehiro A, "Tumor necrosis factor-alpha negatively regulates airway hyperresponsiveness through gamma-delta T cells" 164 (164): 2229-2238, 2001

2 Artis D, "Tumor necrosis factor alpha is a critical component of interleukin 13-mediated protective T helper cell type 2 responses during helminth infection" 190 (190): 953-962, 1999

3 Lee JH, "The levels of CD4+CD25+ regulatory T cells in paediatric patients with allergic rhinitis and bronchial asthma" 148 (148): 53-63, 2007

4 Lin YL, "The functional insufficiency of human CD4+CD25 high T-regulatory cells in allergic asthma is subjected to TNF-alpha modulation" 63 (63): 67-74, 2008

5 Iwasaki M, "TNF-alpha contributes to the development of allergic rhinitis in mice" 112 (112): 134-140, 2003

6 Ling EM, "Relation of CD4+CD25+ regulatory T-cell suppression of allergendriven T-cell activation to atopic status and expression of allergic disease" 363 (363): 608-615, 2004

7 Noval Rivas M, "Regulatory T cells in allergic diseases" 138 (138): 639-652, 2016

8 Pellerin L, "Regulatory T cells and their roles in immune dysregulation and allergy" 58 (58): 358-368, 2014

9 Hartl D, "Quantitative and functional impairment of pulmonary CD4+CD25hi regulatory T cells in pediatric asthma" 119 (119): 1258-1266, 2007

10 Choi EY, "Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats" 47 (47): 134-139, 2009