Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts

다국어 초록 (Multilingual Abstract)

In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichumNakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at 121℃, 1.2 atm for 15 minutes. To confirmeffective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidantactivity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated basedon inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1),tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Eachextract showed strong antioxidant and whitening activity. IC50 values of antioxidant activity from each extract were in orderof 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melaninsynthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A.
distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials forwhitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A.
distichum as a cosmetic material.

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