Osmolarity hypersensitivity of hogl deleted mutants is suppressed by mutation in KSSl in budding yeast Saccharomyces cerevisiae

다국어 초록 (Multilingual Abstract)

An osmosensing mechanism of Saccharomyces cerevisiae involves a mitogen-activated protein kinase (MAPK) cascade (HOG pathway). This study aimed to investigate the response of the yeast to osmotic stress. A mutant strain, in which the HOG1 gene was gene was disrupted by TRP1, was constructed. A spontaneous mutant, named YJY45, which suppresses the osmosensitive growth phenotype of the hog1 deletion mutant, was selected and showed a secondary phenotype of temperature sensitivity on YPD containing 0.5 M NaCl at 37℃. Our data indicate that the spontaneous mutation in YJY45 mutant was mapped KSS1, which is one of the MAPK family. The mutation in KSS1 suppresses the osmolarity-hypersensitive phenotype of the hog1 deletion mutation and restores GPD1 induction. ⓒ 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.