보중익기탕 합 향사육군자탕이 복수암 유발 생쥐 비장의 세포성 면역활성에 미치는 영향에 관한 면역조직화학적 연구 = The Immunohistochemical study for Activation of Cellular Immunity in Spleen of Sarcoma 180 cell injected Mice by Bojungikgitang and Hyangsayukgunjatang

다국어 초록 (Multilingual Abstract)

This study investigated that cellular immunity of spleen is effected by administration of Bojungikgitang and Hyangsayukgunjatang extract (BHE) in Sarcoma 180 cells bearing mice (SBA). The BHE was orally administered daily for 21 days at the dose of 0.743 g/㎏ after Sarcoma 180 cells were intraperitonealiy injected with 1.0×106 cells. The specimen was immunohistochemically stained by Avidin biotin complex methods using monoclonal antibody to L3T4 (CD4), Ly-2 (CD8), IL-2R-α (CD25R-α) and NK-1.1 (CD56) after embedding in paraffin. In BHE treated mice, the number of helper T cells, cytotoxic T cells, and IL-2 receptors are remarkaboy increased in the periarterial lymphatic sheath of white pulp (WP) and penicillary artery and trabecular artery in red pulp (RP). The degree of positive reactions is stronger than SBA. The number of natural killer cells is dramatically increased in marginal zone of WP and penicillary artery of RP and degree of positive reactions is stronger than SBA. Above results show that BHE is effective to increase cellular immunity. It can be considered that the BHE may play a role as efficacious antitumor agent.

목차 (Table of Contents)

• Ⅰ.서론
• Ⅱ.재료 및 방법
• 1.실험동물
• 2.복수암세포
• 3.보중익기탕 합 향사육군자탕 추출물의 제조와 투여
• 4.비장조직 표본 작성
• 5.면역조직화학적 염색
• Ⅲ.결 과
• 1.도움 T 림프구의 분포 변화
• 2.세포독성 T 림프구의 분포 변화
• 3.IL-2 수용체의 분포 변화
• 4.자연살해세포의 분포 변화
• Ⅳ.고 찰
• Ⅴ.결론